Downregulation of TfR1 promotes progression of colorectal cancer via the JAK/STAT pathway

Cancer Management and Research
Can CuiChunyi Hao

Abstract

Background: Colorectal cancer (CRC) is one of the most prevalent gastrointestinal malignancies. The incidence of CRC has been rapidly increasing in China. Transferrin receptor 1 (TfR1) is a key regulator of cellular iron homeostasis. Several studies have demonstrated TfR1 overexpression in a variety of human tumors, but the association between TfR1 and CRC remains unclear. Methods: TfR1 expression was evaluated in six CRC cell lines and tumor tissues. A total of 201 CRC patients were included for immunohistochemistry and 19 pairs of frozen tissues were used for real-time PCR. Cell proliferation, cell cycle, cell migration and invasion, and in vivo carcinogenesis were tested after downregulation of TfR1 by lentivirus. Protein microarray and Western blot analyses were used to explore the underlying mechanisms of TfR1 in CRC. Results: TfR1 expression was higher in CRC tissues than in normal tissues (57.2% vs 22.9%, P<0.001). TfR1 expression was obviously higher in CRC tissues with well differentiation (P=0.008), no lymph node metastasis (P=0.002), no distant metastasis (P=0.006), no vascular invasion (P<0.001) and early TNM stage (P=0.013). CRC patients with TfR1-positive expression had a better survival than those with TfR1-negat...Continue Reading

Citations

Apr 25, 2020·Journal of Basic and Clinical Physiology and Pharmacology·Milad AshrafizadehElham Ghasemipour Afshar
Aug 9, 2020·Cell Biology International·Mazen HasanNupur K Das
Mar 12, 2020·Phytotherapy Research : PTR·Milad AshrafizadehSaeed Samarghandian
Mar 28, 2020·International Journal of Molecular Sciences·Stefania ForcinitiLuigi Laghi
Oct 2, 2019·Cells·Milad AshrafizadehGautam Sethi
Apr 25, 2020·Frontiers in Oncology·Rikki A M BrownPeter J Leedman
Feb 21, 2021·The Journal of Biological Chemistry·Xiaojuan WangWei Liu
Jul 27, 2021·Computational and Mathematical Methods in Medicine·Yunping Lu
Sep 24, 2021·Journal of Neurochemistry·Md JakariaScott Ayton

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Methods Mentioned

BETA
surgical resection
PCR
electrophoresis
Protein Assay
flow cytometry
Profiler
xenograft

Software Mentioned

Image J
SPSS

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