Downregulation of the osmolyte transporters SMIT and BGT1 by AMP-activated protein kinase

Biochemical and Biophysical Research Communications
Carlos MunozFlorian Lang

Abstract

The myoinositol transporter SMIT (SLC5A3) and the betaine/γ-aminobutyric acid (GABA) transporter BGT1 (SLC6A12) accomplish cellular accumulation of organic osmolytes and thus contribute to cell volume regulation. Challenges of cell volume constancy include energy depletion, which compromises the function of the Na(+)/K(+) ATPase leading to cellular Na(+) accumulation and subsequent cell swelling. Energy depletion is sensed by AMP-activated protein kinase (AMPK). The present study explored whether AMPK influences the activity of SMIT and BGT1. To this end, cRNA encoding SMIT or BGT1 was injected into Xenopus oocytes with and without additional injection of wild type AMPK (AMPKα1+AMPKβ1+AMPKγ1), of constitutively active (γR70Q)AMPK (AMPKα1+AMPKβ1+(R70Q)AMPKγ1) or of catalytically inactive (αK45R)AMPK ((K45R)AMPKα1+AMPKβ1+AMPKγ1). Substrate-induced current in dual electrode voltage-clamp experiments was taken as measure of osmolyte transport. As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (I(SMIT)), which was half maximal (K(M)) at ≈7.2μM myoinositol concentration. Furthermore, in BGT1-expressing, but not in water-injected Xenopus oocyte...Continue Reading

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Citations

Jan 21, 2014·Journal of Biochemistry·Miribane Dërmaku-SopjaniMentor Sopjani
Dec 25, 2013·Channels·Florian Lang, Michael Föller
Jul 11, 2014·Biochemical and Biophysical Research Communications·Musaab AhmedFlorian Lang
May 16, 2014·American Journal of Physiology. Renal Physiology·Eva S SchweikhardBirgitta C Burckhardt
Nov 9, 2018·International Journal of Molecular Sciences·Philipp Glosse, Michael Föller

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