Downregulation of the WT1 gene expression via TMPyP4 stabilization of promoter G-quadruplexes in leukemia cells

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Saeedeh Ghazaey ZidanlooJahan-Bakhsh Raoof

Abstract

The WT1 gene is an important oncogene, and its overexpression is considered as an effective target for anticancer therapy. Regulation of its gene transcription is one way for WT1-targeting drug design. Recently, in silico analysis of some oncogene promoters like WT1 showed some guanine-rich regions with the ability to form G-quadruplex structures. Ligands like 5,10,15,20-tetra(N-methyl-4-pyridyl)-porphine (TMPyP4) have predominant effect on G-quadruplex stabilization. The aim of this study was to understand the effect of TMPyP4 on WT1 gene transcription via stabilization of promoter G-quadruplexes. We examined the formation of new G-quadruplex motifs in WT1 promoter in the presence of TMPyP4. In order to understand the nature of its interaction with WT1 promoter quadruplexes, differential pulse voltammetry (DPV), circular dichroism (CD), polyacrylamide gel electrophoresis, electrophoretic mobility shift assay (EMSA), polymerase chain reaction (PCR) stop assays, and quantitative RT-PCR were performed. According to the results, the WT1 promoter can form stable intramolecular parallel G-quadruplexes. In addition, after 48 and 96 h of incubation, 100 μM TMPyP4 reduced the WT1 transcription to 9 and 0.4 %, respectively, compare to c...Continue Reading

References

Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L DaileyN Heintz
Dec 10, 1999·Biochemistry·J Ren, J B Chaires
Sep 13, 2001·Journal of the American Chemical Society·H HanL H Hurley
Jul 20, 2002·Biochemical and Biophysical Research Communications·Pablo Zapata-BenavidesAna M Tari
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam Siddiqui-JainLaurence H Hurley
Feb 13, 2003·Nucleic Acids Research·Daisuke MiyoshiNaoki Sugimoto
Sep 24, 2004·Biochemical and Biophysical Research Communications·Thibault LemarteleurJean-François Riou
Jul 28, 2005·Journal of the American Chemical Society·Sarah RankinStephen Neidle
Jun 21, 2006·Biochemistry·Himesh FernandoShankar Balasubramanian
Jul 18, 2006·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Feb 27, 2007·Nature Chemical Biology·Sunita KumariShankar Balasubramanian
Aug 11, 2007·Leukemia·W GlienkeL Bergmann
Oct 4, 2007·Pflügers Archiv : European journal of physiology·Nicole WagnerKay-Dietrich Wagner
Oct 6, 2007·Nucleic Acids Research·Ren ZhangChun-Ting Zhang
Feb 26, 2008·Biochimie·J L Huppert
Jun 22, 2010·Future Medicinal Chemistry·Danzhou Yang, Keika Okamoto
Mar 2, 2011·Nucleic Acids Research·Alan K Todd, Stephen Neidle
Nov 23, 2011·Bioorganic & Medicinal Chemistry Letters·Thanachai TakaWirote Tuntiwechapikul
May 7, 2013·Physical Chemistry Chemical Physics : PCCP·Ana-Maria Chiorcea-PaquimAna Maria Oliveira-Brett

❮ Previous
Next ❯

Citations

Jul 16, 2020·International Journal of Molecular Sciences·Ilaria CelaLuca Federici
Dec 7, 2019·Journal of Biomolecular Structure & Dynamics·Anju SinghShrikant Kukreti
Feb 27, 2021·Molecular Cancer·Nils KosiolKatrin Paeschke
Mar 13, 2021·BMC Biology·Alessio De MagisKatrin Paeschke
May 19, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jia-Yuan GongZheng Tan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.