Downregulation of tumorogenicity and changes in the actin cytoskeleton of murine hepatoma after irradiation with polychromatic visible and IR light

Photomedicine and Laser Surgery
Nickolay A KnyazevN A Filatova

Abstract

This study evaluated the function and structural consequences of direct exposure of murine hepatoma MH-22a cells to polychromatic polarized light, to determine potential risk of malignancy following irradiation. Visible (VIS) and infrared (IR) light have been actively used for prevention and treatment of complications developed after conventional tumor therapy. However, the safety associated with this irradiation has not been determined. Polychromatic light (480-3400 and 385-750 nm), were used at different doses (4.8-38.4 J/cm(2)) to determine the viability, proliferation, and actin cytoskeleton in vitro by flow cytometry and confocal microscopy. Tumorogenic properties of cells were studied in vivo after transplantation in C3HA mice. Polychromatic light of a wide range of doses did not change the viability and proliferation of cells. After transplantation of cells irradiated with VIS-IR light (4.8 and 9.6 J/cm(2)) and VIS light (38.4 J/cm(2)) the tumor volume was lower in the treated group than in the control group in vivo. Transplantability of the irradiated cells also decreased, whereas survival of tumor-bearing mice increased. Three cell populations with different cytoskeleton structure were identified. After irradiation, th...Continue Reading

References

Apr 1, 1994·Cytometry·K RadosevićJ Greve
Sep 20, 2002·Brazilian Dental Journal·Antonio Luiz Barbosa PinheiroAldo Brugnera
Apr 22, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·William PostenMurad Alam
Dec 17, 2005·Photomedicine and Laser Surgery·Jurema Lisboa Freire de CastroCristina Pacheco Soares
Aug 9, 2011·Nature Reviews. Cancer·Sarah Seton-Rogers

❮ Previous
Next ❯

Software Mentioned

Statistica
ModFit LT

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.