Downregulation of varicella-zoster virus (VZV) immediate-early ORF62 transcription by VZV ORF63 correlates with virus replication in vitro and with latency

Journal of Virology
Susan E HooverJeffrey I Cohen

Abstract

Varicella-zoster virus (VZV) open reading frame 63 (ORF63) protein is expressed during latency in human sensory ganglia. Deletion of ORF63 impairs virus replication in cell culture and establishment of latency in cotton rats. We found that cells infected with a VZV ORF63 deletion mutant yielded low titers of cell-free virus and produced very few enveloped virions detectable by electron microscopy compared with those infected with parental virus. Microarray analysis of cells infected with a recombinant adenovirus expressing ORF63 showed that transcription of few human genes was affected by ORF63; a heat shock 70-kDa protein gene was downregulated, and several histone genes were upregulated. In experiments using VZV transcription arrays, deletion of ORF63 from VZV resulted in a fourfold increase in expression of ORF62, the major viral transcriptional activator. A threefold increase in ORF62 protein was observed in cells infected with the ORF63 deletion mutant compared with those infected with parental virus. Cells infected with ORF63 mutants impaired for replication and latency (J. I. Cohen, T. Krogmann, S. Bontems, C. Sadzot-Delvaux, and L. Pesnicak, J. Virol. 79:5069-5077, 2005) showed an increase in ORF62 transcription compare...Continue Reading

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Citations

Sep 23, 2008·Archives of Virology·Merry LiuAbbas Vafai
Jun 14, 2012·Herpesviridae·Paul R KinchingtonRobert L Hendricks
Jun 23, 2011·Future Virology·Emily EshlemanRandall J Cohrs
Apr 15, 2011·Uirusu·Kyosuke KanaiNaoki Inoue
Feb 11, 2014·Nature Reviews. Microbiology·Leigh ZerboniAnn M Arvin
Jul 26, 2015·Science China. Life Sciences·Wei WangNingShao Xia
Aug 5, 2000·Mayo Clinic Proceedings·T J Liesegang
Aug 2, 2018·The Journal of General Virology·Imane BoumartCatherine Dupuy
May 9, 2014·Journal of Virology·Melissa A VisalliRobert J Visalli
Feb 6, 2020·Frontiers in Cellular and Infection Microbiology·Yangguang LiAnchun Cheng

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