Downregulation of VMP1 confers aggressive properties to colorectal cancer

Oncology Reports
Xian-Zhi GuoMing-Hua Yu

Abstract

Vacuole membrane protein 1 (VMP1) was recently found to be involved in the process of tumor metastasis and is also considered to play a vital role in balancing apoptosis and autophagy. In the present study, the expression of VMP1 in colorectal cancer and matched adjacent non‑cancerous tissues was evaluated by immunohistochemistry (IHC) for studying the role of VMP1 in the process of colorectal cancer. Kaplan‑Meier analysis and the log-rank test were used to calculate the correlation of classic clinicopathological characteristics related to survival and the expression of VMP1. In vitro, a VMP1 stable gene silencing cell model was constructed using a lentiviral vector. The invasive ability and proliferation of colorectal cancer cells were evaluated by Transwell and MTT assays, respectively, and the underlying signaling pathway was explored by western blotting. Additionally, drug susceptibility to cisplatin, oxaliplatin and 5-FU was tested before and after VMP1 knockout. Finally, an animal model was constructed to explore the role of VMP1 in the physiopathologic process of colorectal cancer. Our results indicated that VMP1 showed increased expression in the adjacent non-cancer tissues compared with that in the colorectal cancer ti...Continue Reading

References

Nov 28, 2002·Clinical Colorectal Cancer·Jennifer ReedDomenico Coppola
Jul 29, 2006·Autophagy·Jayanta DebnathGuido Kroemer
Aug 25, 2006·Nature Methods·David E RootDavid M Sabatini
Sep 1, 2007·Nature Cell Biology·Andreas Wodarz, Inke Näthke
Nov 17, 2007·Genes & Development·Noboru Mizushima
Jun 17, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·X-F Huang, J-Z Chen
Dec 1, 2009·Current Opinion in Cell Biology·Candia M KenificJayanta Debnath
Jan 22, 2010·Expert Opinion on Biological Therapy·Sandesh SubramanyaPremlata Shankar
Sep 30, 2010·Journal of Clinical Pathology·Alexandra GiatromanolakiEfthimios Sivridis
Dec 22, 2010·The Journal of Biological Chemistry·Daniel GrassoMaría I Vaccaro
May 24, 2011·Annals of Surgical Oncology·Shi Yu Yang, Marc C Winslet
Aug 11, 2011·Recent Patents on DNA & Gene Sequences·Karina B AcostaPedro D Zapata
Jan 15, 2013·Scientific Reports·Maria I MolejonMaria I Vaccaro
Mar 6, 2013·Journal of Cellular Physiology·Marine GilabertJuan L Iovanna
Dec 25, 2013·Biochemical and Biophysical Research Communications·Qinyi QianWen Gu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Parkinson's Disease & Autophagy (MDS)

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis