PMID: 15223791Jun 30, 2004Paper

Downstream effects of ROCK signaling in cultured human corneal stromal cells: microarray analysis of gene expression

Investigative Ophthalmology & Visual Science
Stephen A K HarveyNirmala SundarRaj

Abstract

Rho-associated coiled-coil-containing protein kinase (ROCK) is a downstream target of Rho GTPase signaling and regulates the assembly of stress fibers. Previous reports indicate that Rho/ROCK signaling is involved in the regulation of several cellular processes, some of which may be cell-type specific and are probably critical to corneal stromal cell activation. The present study identified ROCK-regulated gene expression in corneal stromal cells. Corneal stromal cells derived from eyes of three different donors were cultured to yield the following designated phenotypes: baseline fibroblasts (DMEM with 10% serum), activated fibroblasts (10% serum+bFGF+heparin), and myofibroblasts (1% serum+TGF-beta 1). Cells were exposed to the ROCK inhibitor Y-27632 or vehicle for 12 hours, and transcript levels altered by ROCK inhibition were identified with oligonucleotide microarrays (GeneChips; Affymetrix, Santa Clara, CA). In these phenotypes, Y-27632 caused marked (twofold or more) increases or decreases in 14/4, 12/3, and 15/10 transcripts. In both fibroblast groups Y-27632-treatment increased expression of endothelin receptors and of parathyroid hormone-like hormone. The upregulation of alpha-smooth muscle actin in myofibroblasts was at...Continue Reading

Citations

Oct 4, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Marjan BoermaMartin Hauer-Jensen
Jun 2, 2006·Molecular and Cellular Biology·Daniel R Croft, Michael F Olson
May 23, 2008·American Journal of Physiology. Cell Physiology·Jia Yin, Fu-Shin X Yu
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Mehrnoosh SaghizadehAlexander V Ljubimov
Sep 18, 2013·International Journal of Oncology·Jing WangYisheng Zhong
Mar 8, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Nuno Moura-CoelhoRita Pinto Proença
Feb 16, 2018·Japanese Journal of Ophthalmology·Megumi Honjo, Hidenobu Tanihara

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