Downstream Microvascular Thrombosis in Cortical Venules Is an Early Response to Proximal Cerebral Arterial Occlusion

Journal of the American Heart Association
Jean-Philippe DesillesBenoit Ho-Tin-Noé

Abstract

Previous experimental studies have shown that downstream microvascular thromboinflammation is involved in brain damage from acute ischemic stroke. Using intravital microscopy, we investigated and characterized the sequence of downstream microvascular thromboinflammation in an ischemia/reperfusion acute ischemic stroke model. Rats underwent transient monofilament middle cerebral artery (MCA) occlusion. Cerebral microcirculation in the MCA territory was exposed through a craniotomy and analyzed using real-time intravital imaging coupled with laser Doppler interferometry. Leukocytes, platelets, fibrinogen, and blood-brain barrier permeability were analyzed by intravenous injection of fluorescent antibodies and bovine serum albumin. MCA occlusion induced a sudden and profound drop in downstream microvascular blood flow associated with leukocyte margination in the venous compartment. Leukocyte margination fostered fibrinogen deposition and thrombosis in postcapillary venules. Either in venules or arterioles, blood flow was not fully restored after MCA recanalization. Furthermore, venular thrombi persisted despite MCA recanalization, and leukocyte extravasation continued to develop in venules in association with blood-brain barrier d...Continue Reading

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May 21, 2017·Stroke; a Journal of Cerebral Circulation·Jean-Philippe DesillesBenoit Ho-Tin-Noé

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Citations

Dec 26, 2018·Circulation Research·Vanessa H BraitAnna M Planas
Jan 4, 2020·Annals of Neurology·Alexander M KollikowskiMirko Pham
Sep 23, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adrien Ter SchiphorstCatherine Oppenheim
Sep 2, 2020·Journal of Thrombosis and Haemostasis : JTH·Joachim C MertensJean-Philippe Desilles
Sep 25, 2020·Nature Reviews. Neurology·Guido Stoll, Mirko Pham
Jan 11, 2020·Frontiers in Immunology·Frederik DenormeSimon F De Meyer
Feb 8, 2019·Stroke; a Journal of Cerebral Circulation·Jean-Philippe DesillesBenoît Ho-Tin-Noé
Dec 11, 2020·Circulation Research·Anja NitzscheEric Camerer
Apr 10, 2021·Journal of Thrombosis and Haemostasis : JTH·Maxime GaubertiDenis Vivien
Aug 30, 2021·Translational Stroke Research·Alexander M KollikowskiMichael K Schuhmann
Sep 21, 2021·Stroke; a Journal of Cerebral Circulation·Marie CoutureUNKNOWN ETIS Registry Investigators*
Sep 23, 2021·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Jose Danilo Bengzon DiestroJulian Spears

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Software Mentioned

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Polytec Vibrometer
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