Downstream protein separation by surfactant precipitation: a review

Critical Reviews in Biotechnology
Fadzlie Wong Faizal WongDavid C Stuckey

Abstract

In a conventional protein downstream processing (DSP) scheme, chromatography is the single most expensive step. Despite being highly effective, it often has a low process throughput due to its semibatch nature, sometimes with nonreproducible results and relatively complex process development. Hence, more work is required to develop alternative purification methods that are more cost-effective, but exhibiting nearly comparable performance. In recent years, surfactant precipitation has been heralded as a promising new method for primary protein recovery that meets these criteria and is a simple and cost-effective method that purifies and concentrates. The method requires the direct addition of a surfactant to a complex solution (e.g. a fermentation broth) containing the protein of interest, where the final surfactant concentration is maintained below its critical micelle concentration (CMC) in order to allow for electrostatic and hydrophobic interactions between the surfactant and the target protein. An insoluble (hydrophobic) protein-surfactant complex is formed and backextraction of the target protein from the precipitate into a new aqueous phase is then carried out using either solvent extraction, or addition of a counter-ioni...Continue Reading

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Citations

Dec 4, 2019·Probiotics and Antimicrobial Proteins·Say-Yed Hesameddin TafreshiShohreh Khatami
Jan 15, 2020·Critical Reviews in Biotechnology·Sharifah Fathiyah Sy MohamadWan Mohd Azizi Wan Sulaiman

Related Concepts

Hydrogen-Ion Concentration
Osmolality
Chemical Precipitation
Proteins, Recombinant DNA
Tensides
Chromatography
Critical Illness
Fermentation
Micelles
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