Doxazosin reduces cell proliferation and increases collagen fibers in rat prostatic lobes

Cell and Tissue Research
Luis A JustulinSérgio L Felisbino

Abstract

We investigated the effects of doxazosin (Dox), an alpha-adrenoceptor antagonist used clinically for the treatment of benign prostatic hyperplasia (BPH), on the rat prostatic complex by assessing structural parameters, collagen fiber content, cell proliferation, and apoptosis. Adult Wistar rats were treated with Dox (25 mg/kg per day), and the ventral (VP), dorsolateral, and anterior prostate (AP) regions of the prostate complex were excised at 3, 7, and 30 days after treatment. At 24 h before being killed, the rats were injected once with 5-bromodeoxyuridine (BrdU; thymidine analog) to label mitotically active cells. The prostates were weighed and processed for histochemistry, morphometry-stereology, immunohistochemistry for BrdU, Western blotting for proliferating cell nuclear antigen (PCNA), and the TUNEL reaction for apoptosis. Dox-treated prostate lobes at day 3 presented increased weight, an enlarged ductal lumen, low cubical epithelial cells, reduced epithelial folds, and stretched smooth muscle cells. However, at day 30, the prostates exhibited a weight reduction of approximately 20% and an increased area of collagen and reticular fibers in the stromal space. Dox also reduced epithelial cell proliferation and increased ...Continue Reading

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Citations

Oct 15, 2009·Clinical and Experimental Medicine·Tetsuya ImamuraYoshiki Sugimura
Nov 10, 2012·International Journal of Experimental Pathology·Carolina SaroboSérgio L Felisbino
Feb 16, 2010·Microscopy Research and Technique·Flávia Karina Delella, Sérgio Luis Felisbino
Jun 6, 2009·International Journal of Andrology·Luis A JustulinSérgio L Felisbino
Oct 17, 2012·Life Sciences·Flávia K DelellaSérgio L Felisbino
Jan 25, 2017·Cancers·Damien A Leach, Grant Buchanan
May 12, 2017·Cell Biology International·Flávia Karina DelellaSérgio Luis Felisbino
Apr 5, 2018·Journal of Clinical Medicine·Kenichiro IshiiMasatoshi Watanabe

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