DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes.

Bio-protocol
Rituraj Marwaha, Mahak Sharma

Abstract

Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery and degradation by endo-lysosomes is a tightly regulated process and important for maintaining cellular homeostasis. Cargos like EGF (Epidermal Growth Factor), Dil-LDL (3,3'-Dioctadecylindocarbocyanine-Low Density Lipoprotein), Dextran, DQ-BSA (Dye Quenched-Bovine Serum Albumin) etc., are routinely used by researchers to analyze the role of various proteins in endocytic pathway. Trafficking of DQ-BSA in cells depleted of or over-expressing the gene of interest is a useful assay for identifying the role of various proteins in endocytic trafficking pathway. The protocol describes the DQ-Red BSA trafficking assay that can be used to study endocytic trafficking in various cell types.

References

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Apr 9, 2016·Cellular Logistics·Divya KhatterMahak Sharma
Mar 23, 2017·The Journal of Cell Biology·Rituraj MarwahaMahak Sharma

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Citations

Nov 7, 2018·Nature Cell Biology·Alberto di RonzaMarco Sardiello
Mar 29, 2019·Autophagy·Lorena Fernandez-MosqueraNuno Raimundo
Apr 4, 2019·Frontiers in Immunology·Diego Pérez-StuardoSebastián Reyes-Cerpa
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Dec 8, 2020·Frontiers in Immunology·Diego Pérez-StuardoSebastián Reyes-Cerpa
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Apr 23, 2020·Journal of the American Chemical Society·Ivan PavlinovLeslie N Aldrich
Jul 1, 2020·The Journal of Physical Chemistry Letters·Aditya YadavChayan K Nandi

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