dra-related X adhesins of gestational pyelonephritis-associated Escherichia coli recognize SCR-3 and SCR-4 domains of recombinant decay-accelerating factor.

Infection and Immunity
T PhamB J Nowicki

Abstract

Bacterial adhesins are important virulence factors that allow colonization of the human urogenital tract by Escherichia coli. Adhesins of the Dr family have been found to be more frequently expressed in strains associated with symptomatic urinary tract infections. Because of the high frequency of symptomatic urinary tract infections during pregnancy, we screened E. coli isolates from 64 gestational pyelonephritis patients for the expression of Dr and X adhesins to address their potential virulence roles in this population. Using PCR and primers for the afaB gene, we detected dra-related operons in 17 isolates (27%). On the basis of the lack of hemagglutination of Dr(a-) erythrocytes containing a point mutation in the decay-accelerating factor (DAF) short consensus repeat-3 (SCR-3) domain, 12 of these strains were categorized as classical Dr adhesins. The hemagglutination of O erythrocytes by Dr+ strains was blocked or reduced by a monoclonal antibody to the DAF SCR-3 domain. The remaining five dra-positive strains agglutinated Dr(a-) erythrocytes. Monoclonal antibody to the DAF SCR-3 domain failed to block O-erythrocyte hemagglutination. Adhesins in these strains did not fulfill criteria for Dr hemagglutinins because of the und...Continue Reading

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Citations

Nov 14, 1997·American Journal of Reproductive Immunology : AJRI·B NowickiC Yallampalli
Mar 18, 2006·The Journal of Biological Chemistry·Daniel P O'BrienRichard M Peek
Jul 12, 2008·European Journal of Clinical Investigation·B K DhakalM A Mulvey
Dec 25, 2002·The Journal of Biological Chemistry·Pamela WilliamsSusan Lea
Mar 29, 2006·Journal of Translational Medicine·D L Mager
Jun 27, 2009·BMC Medical Genomics·John W GowAbhijit Chaudhuri
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J M Pérez de la LastraB P Morgan
Aug 13, 2002·The Journal of Biological Chemistry·Heather L GreenstoneEdward A Berger
Mar 18, 2005·Annual Review of Immunology·P R TaylorS Gordon
Jul 28, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J C RiceB J Nowicki
Apr 16, 2005·Clinical Microbiology Reviews·Alain L Servin
Jul 19, 2002·Molecular Microbiology·Cristina P Van LoySteve L Moseley
Nov 28, 2001·Protein Expression and Purification·B ZalewskaJ Kur
Jul 25, 2000·Developmental and Comparative Immunology·L Kuttner-KondoM E Medof
Feb 19, 2000·Current Opinion in Immunology·G LindahlE Johnsson
Mar 6, 1999·Current Opinion in Microbiology·S N AbrahamS Normark
Nov 1, 2005·EcoSal Plus·Chantal Le Bouguénec, James P Nataro

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