Sep 10, 2015

Draft genome of Brugia pahangi: high similarity between B. pahangi and B. malayi

Parasites & Vectors
Yee Ling LauMun-Yik Fong

Abstract

Efforts to completely eradicate lymphatic filariasis from human population may be challenged by the emergence of Brugia pahangi as another zoonotic lymphatic filarial nematode. In this report, a genomic study was conducted to understand this species at molecular level. After blood meal on a B. pahangi-harbouring cat, the Aedes togoi mosquitoes were maintained to harvest infective third stage larvae, which were then injected into male Mongolian gerbils. Subsequently, adult B. pahangi were obtained from the infected gerbil for genomic DNA extraction. Sequencing and subsequently, construction of genomic libraries were performed. This was followed by genomic analyses and gene annotation analysis. By using archived protein sequences of B. malayi and a few other nematodes, clustering of gene orthologs and phylogenetics were conducted. A total of 9687 coding genes were predicted. The genome of B. pahangi shared high similarity to that B. malayi genome, particularly genes annotated to fundamental processes. Nevertheless, 166 genes were considered to be unique to B. pahangi, which may be responsible for the distinct properties of B. pahangi as compared to other filarial nematodes. In addition, 803 genes were deduced to be derived from W...Continue Reading

Mentioned in this Paper

Calcinus elegans
Conserved Synteny
Metabolic Process, Cellular
Cyartonema elegans
Severe Acute Respiratory Syndrome
Genee-Wiedemann Syndrome
Short Interspersed Nucleotide Elements
Study
IL12A
Coleonyx elegans

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