Draft genome sequence of KPC-2-producing Pseudomonas aeruginosa recovered from a bloodstream infection sample in Brazil

Journal of Global Antimicrobial Resistance
Ivson Cassiano de Oliveira SantosAna Paula D'Alincourt Carvalho-Assef

Abstract

Klebsiella pneumoniae carbapenemase (KPC) is the most widespread carbapenemase in Enterobacteriaceae in Brazil. Although its presence is not common in Pseudomonas aeruginosa, it has been increasingly reported. Here we report a draft genome sequence of a KPC-producing P. aeruginosa strain recovered from a bloodstream infection sample in Brazil. The antimicrobial susceptibility of KPC-producing P. aeruginosa CCBH17348 was evaluated by the disk diffusion method, Etest and broth microdilution. Carbapenemase production was confirmed by colorimetric assay (Carba NP) and PCR. Genomic DNA was sequenced by Illumina MiSeq sequencing and was assembled using the A5-Miseq pipeline, and gene annotation was performed using RAST 2.0. The database ResFinder 2.1, CRISPRFinder and MLST website were used to identify resistance genes, clustered regularly interspaced short palindromic repeats (CRISPRs) and sequence types (STs), respectively. Isolate CCBH17348 was considered multidrug-resistant, was susceptible to fluoroquinolones, gentamicin and polymyxin, and belonged to a newly described ST (ST2584), carrying an IncQ1 plasmid with blaKPC-2 and aph(3')-VI genes. Other genes associated with resistance and virulence found in the genome were blaOXA-50...Continue Reading

Citations

Feb 28, 2020·Clinical Microbiology Reviews·Karen Bush, Patricia A Bradford
Aug 20, 2020·Expert Review of Anti-infective Therapy·Juan Carlos García-BetancurMaría Virginia Villegas
Jul 12, 2019·Frontiers in Pharmacology·Renata LimaVictor M Balcão
Apr 1, 2021·Journal of Global Antimicrobial Resistance·Daniela Cristina TartariThaís Cristine Marques Sincero

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