Drainage of fluorescent liposomes from the vitreous to cervical lymph nodes via conjunctival lymphatics

Ophthalmic Research
Serge CameloYvonne de Kozak

Abstract

The use of liposomes as carriers for the delivery of biologically active molecules into the eye is of major interest. Indeed, encapsulation of biologically active molecules in liposomes may increase their bioavailability and may induce a sustained release, thus avoiding repeated intraocular injections and reducing side effects. We describe here the fate of rhodamine-conjugated liposomes (Rh-Lip) injected into the vitreous of normal Lewis rats. Twenty-four hours after intravitreal injection fluorescent liposomes were detected in the vitreous, the inner layer of the retina and to a lesser extent in the anterior segment of the eye. In addition, numerous Rh-Lip were also observed in the episclera and conjunctival stroma, in conjunctival lymphatic vessels and cervical lymph nodes (LN) draining the conjunctiva and the eye. In the LN, Rh-Lip were taken up by resident macrophages adjacent to CD4+ and CD8+ T cells. Thus, intravitreal injection of anti-inflammatory drugs loaded in liposomes could modulate the ocular immune microenvironment. In addition the passage of drugs into the cervical LN could alter the immune status of these LN and contribute to the regulation of intraocular inflammation. Our results suggest that this phenomenon s...Continue Reading

Citations

Feb 24, 2009·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Serge CameloYvonne de Kozak
Nov 27, 2009·Journal of the Royal Society, Interface·Wiebke Ulbrich, Alf Lamprecht
Apr 15, 2011·Journal of Drug Delivery·Gyan P MishraAshim K Mitra
Apr 24, 2012·Journal of Ophthalmology·Shintaro NakaoTatsuro Ishibashi
Jun 17, 2014·Expert Opinion on Drug Delivery·Sachin S ThakurHarendra S Parekh
Feb 1, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amélie Bochot, Elias Fattal
Apr 20, 2010·Progress in Retinal and Eye Research·M El SanharawiF Behar-Cohen
Jun 2, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Laure LajavardiAmélie Bochot
Mar 29, 2011·Annales pharmaceutiques françaises·A BochotE Fattal
Nov 12, 2019·Anatomia, histologia, embryologia·İsmail Hakkı NurHaluk Testereci
Mar 7, 2021·International Journal of Molecular Sciences·Seita MorishitaTsunehiko Ikeda
Jul 3, 2021·Biology·Mariela Subileau, Daniel Vittet

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