Drainage of Tumor-Derived DNA into Sentinel Lymph Nodes in Breast Cancer Patients

Pathology Oncology Research : POR
Yukiko MiyamuraShinzaburo Noguchi

Abstract

Circulating tumor DNA (ctDNA) is released from cancer cells by apoptosis or other mechanisms, and as tumor tissue contains both blood and lymphatic vessels, ctDNA can spread to local lymph nodes (LNs). We aimed to detect the tumor-derived free DNA in metastasis-free LNs in patients with breast cancers harboring the PIK3CA-H1047R mutation. One hundred twenty-three patients were evaluated and the PIK3CA-H1047R mutation was assayed in sentinel LNs (SLNs), non-SLNs without metastasis, and serum by digital PCR. The mutant DNA was more frequent in metastasis-free SLNs (21.6%) than in metastasis-free non-SLNs (8.6%; P = 0.038), and patients with mutation-positive SLNs were more likely to be positive for serum mutant DNA. Apoptosis in primary breast tumors was determined by TUNEL assay. The apoptotic index was significantly higher (P = 0.003) in patients with mutation-positive SLNs without metastasis (mean, 1.17%) than those with mutation-negative SLNs without metastasis (mean, 0.79%). It was also significantly higher (P = 0.006) in those with mutation-positive serum (mean, 1.41%) than in those with mutation-negative serum (mean, 0.86%). Furthermore, fragment size of PIK3CA-H1047R mutant DNA in metastatic-free SLN lysate used for the o...Continue Reading

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Citations

Nov 30, 2019·Nature Reviews. Cancer·Gautier FollainJacky G Goetz

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Methods Mentioned

BETA
biopsy
PCR
nucleic acid amplification
dissection
Assay
chip

Software Mentioned

QuantStudio
QuantStudioTM 3D AnalysisSuite
GraphPad
GraphPad Prism

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