DRAM1 regulates the migration and invasion of hepatoblastoma cells via autophagy-EMT pathway

Oncology Letters
Chao ChenGuo-Qiang Zhou

Abstract

DNA-damage regulated autophagy modulator 1 (DRAM1) is known as a target of TP53-mediated autophagy, and has been reported to promote the migration and invasion abilities of glioblastoma stem cells. However, the precise contribution of DRAM1 to cancer cell invasion and migration, and the underlying mechanisms remain unclear. In the present study, small interfering (si)RNA or short hairpin RNA mediated knockdown of DRAM1 was performed in hepatoblastoma cells and the migration and invasion abilities were detected in vitro and in vivo. To investigate the underlying mechanisms, western blotting and immunofluorescence were used to detect the expression of autophagy-associated proteins and epithelial-mesenchymal-transition (EMT)-associated markers. The results showed that DRAM1 knockdown by specific siRNA abrogated cell autophagy, as well as inhibited the migration and invasion of HepG2 cells in Transwell assays, which may be reversed by rapamycin treatment. In addition, DRAM1 knockdown increased the expression of E-Cadherin while decreased the expression of vimentin in HepG2 cells, which was also be reversed by rapamycin treatment. Taken together, these results suggest that DRAM1 is involved in the regulation of the migration and inv...Continue Reading

References

Dec 2, 2000·Science·D J Klionsky, S D Emr
Dec 8, 2000·The Oncologist·C E HerzogF Eftekhari
Aug 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anil DarbariKathleen B Schwarz
Nov 17, 2005·Oncogene·Joseph R Testa, Philip N Tsichlis
Nov 15, 2006·Autophagy·Diane CrightonKevin M Ryan
Mar 3, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Udayan ApteSatdarshan P S Monga
Apr 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xinping TanSatdarshan P S Monga
Jun 26, 2008·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Val CatanzariteJennifer Willert
Jan 21, 2009·Cell Research·Jian XuRik Derynck
Feb 25, 2009·Pediatric Radiology·Fred E AvniMarie Cassart
Apr 4, 2009·The American Journal of Pathology·Michael W Klymkowsky, Pierre Savagner
Jul 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wolfgang HartmannTorsten Pietsch
Sep 16, 2009·Human Pathology·Dolores López-TerradaBarbara B Knowles
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisR Ian Freshney
Oct 23, 2010·Molecular Cell·Guido KroemerBeth Levine
Nov 16, 2011·Autophagy·Li Yen MahKevin M Ryan
May 15, 2012·Cell Cycle·Robin L MacintoshKevin M Ryan
May 23, 2013·PloS One·Xing-Ding ZhangZheng-Hong Qin
Jul 3, 2013·Nature Cell Biology·Patricia BoyaPatrice Codogno
May 8, 2014·Gene Expression·Satdarshan Paul Singh Monga
Jun 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Lei QiangYu-Ying He
Apr 4, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Guangbi LiPin-Lan Li
May 30, 2015·Molecular Oncology·Myriam CatalanoSabrina Di Bartolomeo
Jan 4, 2017·Gastroenterology·Daniela SiaJosep M Llovet

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Methods Mentioned

BETA
biopsy
electrophoresis
surgical resection

Software Mentioned

Image J
SPSS
GraphPad Prism
GraphPad

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