Dec 1, 1992

Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli

Journal of Bacteriology
C A BallR C Johnson

Abstract

Fis is a small basic DNA-binding protein from Escherichia coli that was identified because of its role in site-specific DNA recombination reactions. Recent evidence indicates that Fis also participates in essential cell processes such as rRNA and tRNA transcription and chromosomal DNA replication. In this report, we show that Fis levels vary dramatically during the course of cell growth and in response to changing environmental conditions. When stationary-phase cells are subcultured into a rich medium, Fis levels increase from less than 100 to over 50,000 copies per cell prior to the first cell division. As cells enter exponential growth, nascent synthesis is largely shut off, and intracellular Fis levels decrease as a function of cell division. Fis synthesis also transiently increases when exponentially growing cells are shifted to a richer medium. The magnitude of the peak of Fis synthesis appears to reflect the extent of the nutritional upshift. fis mRNA levels closely resemble the protein expression pattern, suggesting that regulation occurs largely at the transcriptional level. Two RNA polymerase-binding sites and at least six high-affinity Fis-binding sites are present in the fis promoter region. We show that expression o...Continue Reading

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Mentioned in this Paper

Gene Expression Regulation, Bacterial
Fluctuation
Alkalescens-Dispar Group
DNA-Directed RNA Polymerase
RNA Polymerase Assembly Pathway
Protoplasm
Transcription, Genetic
Cell Division
Cell Process Structure
Recombination, Genetic

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