Dramatic co-activation of WWOX/WOX1 with CREB and NF-kappaB in delayed loss of small dorsal root ganglion neurons upon sciatic nerve transection in rats.

PloS One
Meng-Yen LiShur-Tzu Chen

Abstract

Tumor suppressor WOX1 (also named WWOX or FOR) is known to participate in neuronal apoptosis in vivo. Here, we investigated the functional role of WOX1 and transcription factors in the delayed loss of axotomized neurons in dorsal root ganglia (DRG) in rats. Sciatic nerve transection in rats rapidly induced JNK1 activation and upregulation of mRNA and protein expression of WOX1 in the injured DRG neurons in 30 min. Accumulation of p-WOX1, p-JNK1, p-CREB, p-c-Jun, NF-kappaB and ATF3 in the nuclei of injured neurons took place within hours or the first week of injury. At the second month, dramatic nuclear accumulation of WOX1 with CREB (>65% neurons) and NF-kappaB (40-65%) occurred essentially in small DRG neurons, followed by apoptosis at later months. WOX1 physically interacted with CREB most strongly in the nuclei as determined by FRET analysis. Immunoelectron microscopy revealed the complex formation of p-WOX1 with p-CREB and p-c-Jun in vivo. WOX1 blocked the prosurvival CREB-, CRE-, and AP-1-mediated promoter activation in vitro. In contrast, WOX1 enhanced promoter activation governed by c-Jun, Elk-1 and NF-kappaB. WOX1 directly activated NF-kappaB-regulated promoter via its WW domains. Smad4 and p53 were not involved in the ...Continue Reading

References

Feb 7, 2001·The Journal of Biological Chemistry·S PugazhenthiJ E Reusch
Jun 8, 2000·The Journal of Comparative Neurology·T TandrupR E Coggeshall
Sep 29, 2000·Biochemical Pharmacology·J HamB Shah
Dec 12, 2001·Neurochemistry International·Jih Ing Chuang, Shur Tzu Chen
Jan 7, 2003·The Journal of Biological Chemistry·Nan-Shan ChangAmy Ensign
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·S P Persengiev, M R Green
May 14, 2004·Journal of the Neurological Sciences·Kevin D Barron
Dec 22, 2004·The Journal of Comparative Neurology·Li-Ya Qiao, Margaret A Vizzard
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Feng-Jie LaiHamm-Ming Sheu
Oct 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Feb 14, 2006·The Journal of Biological Chemistry·Dan LuTsonwin Hai
Feb 16, 2006·Journal of Neurochemistry·Felicia Yu Hsuan Teng, Bor Luen Tang
Mar 16, 2006·Current Opinion in Neurology·Ying-Chao Chang, Chao-Ching Huang
May 24, 2006·Progress in Neurobiology·Gennadij Raivich, Axel Behrens
Jul 5, 2006·Cancer Research·Haiyan R QinKay Huebner
Dec 5, 2006·Seminars in Cancer Biology·David I SmithDamon S Perez
Dec 5, 2006·Trends in Molecular Medicine·Nan-Shan ChangHamm-Ming Sheu
Dec 21, 2006·Cancer Research·Eugenio GaudioRami I Aqeilan
Apr 27, 2007·Journal of Cellular Physiology·Rami I Aqeilan, Carlo M Croce
May 29, 2007·Neurochemistry International·Jae-Hyuk YiRaghu Vemuganti
Nov 30, 2007·Movement Disorders : Official Journal of the Movement Disorder Society·Kambiz N AlavianHorst H Simon
Jul 16, 2008·Journal of Hepatology·Ileana AdercaLewis R Roberts

❮ Previous
Next ❯

Citations

Jul 26, 2014·Journal of Physiology and Biochemistry·Kyaw Thu MoeMeng Cheong Wong
Jul 25, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ricco LindnerSimone Di Giovanni
Jul 16, 2015·The European Journal of Neuroscience·Anand KrishnanDouglas W Zochodne
May 9, 2012·The Journal of Pain : Official Journal of the American Pain Society·Maike KanngiesserIrmgard Tegeder
Oct 13, 2015·Free Radical Biology & Medicine·Lucie ValekIrmgard Tegeder
Jul 5, 2013·International Journal of Cancer. Journal International Du Cancer·Chung-Ji LiuKuo-Wei Chang
Jan 4, 2017·International Journal of Molecular Sciences·Paola MaroniMaria Alfonsina Desiderio
Dec 10, 2014·Experimental Biology and Medicine·Yao ChangNan-Shan Chang
Oct 25, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jose Vicente Torres-PérezAngelika Varga
Mar 26, 2013·International Journal of Molecular Medicine·Junjie QuXiaoping Wan
Jun 25, 2016·The Journal of Biological Chemistry·Shenq-Shyang HuangNan-Shan Chang
May 12, 2020·Experimental Biology and Medicine·K KoślaA K Bednarek
Jan 8, 2011·Aging·Subhan DudekulaNan-Shan Chang
Nov 30, 2014·Experimental Biology and Medicine·Shin-Yi LiuYu-Jen Chen
Dec 1, 2013·Translational Respiratory Medicine·Qunying HongNan-Shan Chang
Dec 13, 2019·Molecular Brain·Hyun Jin KimJoung-Hun Kim
Mar 25, 2015·Experimental Biology and Medicine·Nan-Shan Chang
Dec 22, 2016·Frontiers in Cell and Developmental Biology·Li-Jin HsuNan-Shan Chang
Oct 30, 2018·Frontiers in Oncology·Mayur Tanna, Rami I Aqeilan
Oct 5, 2018·BMC Biology·Florian BoninKeltouma Driouch
Aug 23, 2017·Biomedicines·Federica BegalliGuido Franzoso
Aug 31, 2018·Frontiers in Neuroscience·Chan-Chuan LiuNan-Shan Chang
Dec 1, 2013·Translational Respiratory Medicine·Szu-Jung ChenNan-Shan Chang
Jan 10, 2018·Experimental Biology and Medicine·Shenq-Shyang Huang, Nan-Shan Chang
Jun 15, 2010·Experimental Biology and Medicine·Jean-Yun ChangNan-Shan Chang
Jan 18, 2015·Experimental Biology and Medicine·Jui-Yen LoLi-Jin Hsu
May 6, 2021·Cells·Karim TaouisFrançois Lallemand
Aug 8, 2021·Cells·Che-Yu HsuNan-Shan Chang

❮ Previous
Next ❯

Methods Mentioned

BETA
FRET
confocal microscopy
flow cytometry
Co-Immunoprecipitation
immunoprecipitation
transmission electron microscopy
Polymerase Chain Reaction
fluorescence-activated cell sorting
Genefector
transfection

Software Mentioned

Pro
Image
Excel
ImagePro

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.