Dried Blood Spot Self-Sampling by Guardians of Children With Epilepsy Is Feasible: Comparison With Plasma for Multiple Antiepileptic Drugs

Therapeutic Drug Monitoring
Camilla LinderAnton Pohanka

Abstract

Dried blood spot (DBS) is an attractive matrix alternative to plasma for the measurement of antiepileptic drug concentrations with the possibility of self-sampling at home. The aim of this study was to evaluate whether DBS concentrations from a children population could be used as an alternative to plasma concentrations in a clinical routine laboratory. Children with epilepsy using carbamazepine (CBZ), lamotrigine (LTG), levetiracetam (LEV), or valproic acid (VPA) had capillary blood collected for routine plasma analysis. DBS samples were collected by guardians or nurses, and the quality of sampling was compared between the groups. DBS samples were analyzed with liquid chromatography-tandem mass spectrometry methods and plasma samples with immunochemical methods. In the comparison between DBS and plasma concentrations, previously analyzed sample data were pooled with data in this study and resulted in 190 comparison pairs. A bioanalytical cross-validation according to European Medicines Agency was performed. Clinicians evaluated the results to understand if a DBS concentration was linked to a different clinical dose recommendation for the patient in comparison with plasma concentrations. Comparison of DBS sample quality showed ...Continue Reading

References

Nov 23, 2006·Epilepsia·Philip N PatsalosJosemir W Sander
Nov 19, 2010·Bioanalysis·Gary Emmons, Malcom Rowland
Jul 14, 2011·Bioanalysis·Philip TimmermanJohn Smeraglia
Apr 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Liliane RhodenRafael Linden
Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Apr 22, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Daniela MilosheskaTomaž Vovk
Nov 18, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Camilla LinderAnton Pohanka
Dec 1, 2017·Clinical Chemistry·Jeffrey D FreemanEllen K Silbergeld
Dec 6, 2017·Expert Opinion on Drug Metabolism & Toxicology·Sofie VelgheChristophe Stove
Apr 7, 2018·Analytical and Bioanalytical Chemistry·Ricardo NetoFlorian Lapierre
Jun 30, 2018·Therapeutic Drug Monitoring·Philip N PatsalosDave J Berry

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Citations

Feb 15, 2020·Expert Opinion on Drug Metabolism & Toxicology·Cecilie Johannessen LandmarkPhilip N Patsalos
Nov 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karina Sommerfeld-KlattaFranciszek K Główka

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