Dried Blood Spots: A Tool to Ensure Broad Access to Hepatitis C Screening, Diagnosis, and Treatment Monitoring

The Journal of Infectious Diseases
Alexandre SoulierStéphane Chevaliez

Abstract

With the advent of highly efficient antiviral therapies for hepatitis C virus (HCV) infection, providing broad access to diagnosis and care is needed. The dried blood spot (DBS) technique can be used to collect, store, and ship whole-blood specimens. Our goal was to assess the performance of standardized HCV diagnostic and monitoring tools in the analysis of DBS. Serum specimens and whole-blood specimens collected using the DBS technique from >500 patients were tested for virological markers used to diagnose and monitor HCV infection. Enzyme immunoassay detection of anti-HCV antibodies in specimens from DBS was reliable after establishment of a new signal-to-cutoff ratio. HCV RNA was detected DBS from the vast majority of patients with active replication, but HCV RNA levels were substantially lower than in serum specimens, implying that only the presence or absence of HCV RNA or changes in the HCV RNA level should be taken into consideration for therapy. Detection of HCV core antigen in specimens from DBS was not a sensitive marker of chronic HCV infection. HCV genotype determination was possible in the vast majority of DBS. This study shows that whole-blood specimens collected using the DBS technique can be confidently used to...Continue Reading

References

Jun 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Magali Bouvier-AliasJean-Michel Pawlotsky
Jun 4, 2010·Journal of Clinical Microbiology·Katrin SteinmetzerEugen Ermantraut
Aug 12, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Johannes ViljoenUNKNOWN World Health Organization/ANRS 1289 Kesho Bora Study Group
Sep 22, 2011·Journal of Viral Hepatitis·A HatzakisH L A Janssen
Sep 8, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc G GhanyUNKNOWN American Association for Study of Liver Diseases
Nov 23, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Khayriyyah Mohd HanafiahSteven T Wiersma
Mar 23, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·C P U BrandãoL M Villar
Oct 17, 2013·American Journal of Human Biology : the Official Journal of the Human Biology Council·Thomas W McDade
Dec 18, 2013·Journal of Hepatology·UNKNOWN European Association for Study of Liver
Mar 19, 2014·Gastroenterology·Jean-Michel Pawlotsky
Jun 29, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stéphane ChevaliezJean-Michel Pawlotsky

❮ Previous
Next ❯

Citations

Oct 22, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Michel Pawlotsky
Sep 20, 2016·Journal of Hepatology·Philippa J Easterbrook, UNKNOWN WHO Guidelines Development Group
Sep 27, 2016·Journal of Hepatology·UNKNOWN European Association for the Study of the Liver. Electronic address: easloffice@easloffice.eu
Aug 9, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Brunna Lemos Crespo MarquesLivia Melo Villar
Jan 29, 2017·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Lisa C MartialAlbert Batalla
Feb 7, 2017·Journal of Medical Virology·Geane Lopes FloresLivia Melo Villar
Mar 14, 2018·The Journal of Infectious Diseases·Francois M J LamouryUNKNOWN LiveRLife Study Group
Nov 2, 2017·Expert Review of Molecular Diagnostics·Jason GrebelyJordan J Feld
Jul 4, 2018·The American Journal of Tropical Medicine and Hygiene·Mark D Lim
Aug 30, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Emma DayUNKNOWN International Network on Hepatitis in Substance Users (INHSU)
Nov 26, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Andrea LombardiUNKNOWN ESCMID Study Group for Viral Hepatitis (ESGVH)
Dec 26, 2018·Expert Review of Anti-infective Therapy·Mauro ViganòAnna Linda Zignego
Apr 6, 2019·Expert Review of Anti-infective Therapy·Stéphane Chevaliez
Sep 7, 2019·Viruses·Mia J BiondiJordan J Feld
Sep 28, 2016·World Journal of Gastroenterology : WJG·Belinda K MössnerDorte Kinggaard Holm
Nov 30, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Zameer MohamedMaud Lemoine
Feb 15, 2019·Journal of Clinical Microbiology·Jenna WeberBenjamin A Pinsky
Jan 23, 2018·Open Forum Infectious Diseases·Alastair HeffernanSimon D Taylor-Robinson
Sep 4, 2015·The Journal of Infectious Diseases·Mark Thursz, Karine Lacombe
Oct 20, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan B ParrGavin Cloherty
Nov 18, 2018·BMC Research Notes·Sebastien KenmoeRichard Njouom
May 23, 2017·Journal of the International AIDS Society·Léa DuchesneKarine Lacombe
Apr 11, 2018·Journal of the International AIDS Society·Slim FouratiNiklas Luhmann
Jan 26, 2020·Journal of Viral Hepatitis·Tram Hong TranYoann Madec
Jul 4, 2020·Open Forum Infectious Diseases·Stéphane ChevaliezStéphanie Dominguez
May 8, 2018·Journal of Immunoassay & Immunochemistry·Helena Medina CruzLivia Melo Villar
Jun 18, 2019·Therapeutic Advances in Infectious Disease·Carmen de MendozaAmalia Díez
Mar 27, 2020·Frontiers in Microbiology·Edouard TuaillonPhilippe Van de Perre
Oct 2, 2019·The Indian Journal of Medical Research·Premashis Kar
Jan 30, 2021·Analytical Methods : Advancing Methods and Applications·Vigneswaran NarayanamurthyFahmi Samsuri
Oct 14, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Samantha J ShepherdRory N Gunson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
C P U BrandãoL M Villar
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Jean-Michel Pawlotsky
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Hayley A CroomKim M Wilson
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
E Kainne DokuboKimberly Page
© 2021 Meta ULC. All rights reserved