Dried Urine Microsampling Coupled to Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the Analysis of Unconjugated Anabolic Androgenic Steroids.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Michele ProttiLaura Mercolini

Abstract

Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation ≤1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.

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Jun 13, 2020·Bioanalysis·Michele ProttiLaura Mercolini

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Citations

Nov 14, 2020·Drug Testing and Analysis·Mario ThevisHans Geyer
Apr 20, 2021·The Journal of Steroid Biochemistry and Molecular Biology·David J HandelsmanRichard A Anderson
Jul 12, 2021·Journal of Pharmaceutical and Biomedical Analysis·Michele ProttiLaura Mercolini

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