PMID: 18187861Jan 12, 2008Paper

"Drop-and-Sip" fluid handling technique for the reagent-release capillary (RRC)-based capillary-assembled microchip (CAs-CHIP): sample delivery optimization and reagent release behavior in RRC

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Terence G HenaresHideaki Hisamoto

Abstract

The experimental conditions of the sample delivery inside the reagent-release capillary-based capillary-assembled microchip (RRC-based CAs-CHIP) were optimized and the reagent release procedure in the RRC is discussed. Recently, our group introduced the basic concept of the "drop-and-sip" fluid handling technique (Anal. Chem., 2007, 79, 908). A microliter volume of sample solution is dropped on the inlet hole and is sipped into another hole, producing a sample plug flow in the main poly(dimethyl siloxane) (PDMS) channel, concurrently filling each sensing capillary that faces the main PDMS channel. However, the detailed evaluation of the successful sample delivery condition and the reagent release behavior in the RRC has not been fully discussed. Under our experimental conditions, ca. 0.6 - 2.4 s of sample plug-RRC contact time allowed the successful sample introduction into the RRC by capillary force without any reagent leakage or disturbance of the sample plug flow. On the other hand, reagent release behavior inside the RRC is governed by both convective and diffusive mass transport, which leads to a faster mixing time of the sample with reagents immobilized inside the RRC compared to that expected from the simple diffusion al...Continue Reading

References

Apr 2, 2005·Analytical Chemistry·Hideaki HisamotoShigeru Terabe
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Greg C Randall, Patrick S Doyle
Apr 28, 2006·Analytical and Bioanalytical Chemistry·Hideaki HisamotoShigeru Terabe
Jun 15, 2006·Analytical Chemistry·Petra S DittrichAndreas Manz
Dec 21, 2006·Lab on a Chip·Martin ZimmermannEmmanuel Delamarche
Aug 29, 2007·Analytica Chimica Acta·Hideaki HisamotoShigeru Terabe

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Citations

Apr 24, 2008·Analytical and Bioanalytical Chemistry·Terence G HenaresHideaki Hisamoto
Jan 11, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Erina TsutsumiHideaki Hisamoto
Aug 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Terence G HenaresHideaki Hisamoto
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Terence G HenaresHideaki Hisamoto

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