Drop-by-drop chemical reaction and sample introduction for capillary electrophoresis

The Analyst
Fengming ChenKatsumi Uchiyama

Abstract

In this paper, we report a novel sample introduction and chemical reaction strategy by drop-by-drop inkjet injection for an electrophoretically mediated microanalysis (EMMA). This method makes it possible to achieve an on-line introduction of reactant solutions by alternately ejecting small plugs, with an overlapping region of the plugs for mixing the reactants by electrophoresis, supporting chemical reactions, followed by electrophoretic separation of the final compounds. As a proof-of-concept of the method, the EMMA of an inkjetted mixture of 4-fluoro-7-nitrobenzofurazan (NBD-F) and amino acids was carried out as a model chemical reaction. The product NBD-amino acids were quantified by detection with laser induced fluorescence. The optimal conditions for the procedure were: inkjet driving voltage: +40-44 V; pulse width: 20-24 μs; drop-by-drop injection of reactant solutions: alternately 2 drops × 25 times for the amino acid solution and the NBD-F solution; zone overlapping voltage and time: 3 kV and 2 s; incubation time after overlapping: 5 min; separation voltage: 18 kV. Under the optimized conditions, a significant enhancement in sensitivity and a sensitive quantitative analysis were realized. The results obtained were comp...Continue Reading

References

Sep 11, 1992·Journal of Chromatography·J Bao, F E Regnier
Oct 1, 1993·Analytical Chemistry·B J HarmonF E Regnier
Apr 7, 2004·Journal of Chromatography. a·Sona NovákováJos Hoogmartens
Oct 3, 2006·Journal of Chromatography. a·Katerina PapezováZdenek Glatz
Oct 15, 2009·Analytical Chemistry·R Michael Verkouteren, Jennifer R Verkouteren
Nov 17, 2012·Analytical Chemistry·Hulie ZengKatsumi Uchiyama
Jun 4, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xu WangAnn Van Schepdael
Jun 12, 2013·Journal of Chromatography. a·L Asensi-BernardiM J Medina-Hernández
Oct 31, 2013·Electrophoresis·Xu WangAnn Van Schepdael

❮ Previous
Next ❯

Citations

Sep 5, 2015·Electrophoresis·Pranov RamanaAnn Van Schepdael
Oct 6, 2015·Electrophoresis·Véréna PoinsotFrançois Couderc
Dec 14, 2016·Journal of Separation Science·Alain Wuethrich, Joselito P Quirino
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc
Aug 15, 2017·Analytical Chemistry·Weifei ZhangKatsumi Uchiyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.