Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose-Response Characteristics System, Background and Examples

Micromachines
Jialan CaoG Alexander Groß

Abstract

Droplet-based microfluidics is a versatile tool to reveal the dose-response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose-response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose-response data for 1D and 2D dose-response screenings are presented.

References

Mar 1, 1979·The American Journal of Sports Medicine·J R Andrews
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·P F Wheat
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karsten ZenglerMartin Keller
Sep 3, 2005·Nature Reviews. Microbiology·Søren J SørensenStefan Wuertz
Oct 1, 2005·Applied Microbiology and Biotechnology·Wolfgang LeuchtenbergerKarlheinz Drauz
Feb 20, 2007·International Journal of Antimicrobial Agents·Matthew E Falagas, Ioannis A Bliziotis
Jul 28, 2007·Applied Microbiology and Biotechnology·Patricia S VaryDieter Jahn
Feb 22, 2008·Nature·Kate E JonesPeter Daszak
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Aug 26, 2009·Lab on a Chip·Adam R AbateDavid A Weitz
Dec 22, 2009·Molecular BioSystems·Yolanda Schaerli, Florian Hollfelder
Mar 3, 2011·PloS One·Jihyang ParkXiaoxia Nina Lin
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Mar 17, 2012·Lab on a Chip·Krzysztof ChurskiPiotr Garstecki
May 15, 2013·Nature Reviews. Microbiology·Joseph A LemireRaymond J Turner
Aug 21, 2013·Biotechnology and Bioengineering·Thomas C ScanlonKarl E Griswold
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·P Gokulakrishnan, Jess Vergis
Jun 19, 2015·Nature·Corie Lok
Jun 30, 2015·Nature Reviews. Microbiology·Peter J Rutledge, Gregory L Challis
Jan 14, 2016·Lab on a Chip·Songzi KouI-Ming Hsing
Jan 25, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L JiangJ Bibette
May 24, 2016·Lab on a Chip·Tomasz S KaminskiPiotr Garstecki
Mar 2, 2018·Environmental Pollution·Maria Arias-AndresHans-Peter Grossart
Apr 8, 2018·Environment International·Célia M ManaiaOlga C Nunes
Jul 22, 2018·SLAS Technology·C SurretteC M Puleo
May 31, 2019·Microbiology Resource Announcements·Rodney Owusu-DarkoElna M Buys
Jun 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jared KehePaul C Blainey

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