Droplet digital PCR for quantification of ITGA6 in a stool mRNA assay for the detection of colorectal cancers

World Journal of Gastroenterology : WJG
Elizabeth HerringJean-François Beaulieu

Abstract

To investigate the use of droplet digital polymerase chain reaction (ddPCR) for detecting host mRNA markers in stools as a non-invasive test for colorectal cancer screening. ddPCR and quantitative PCR were compared side by side for their performance in the detection of ITGA6 and ITGA6A transcripts in stool samples obtained from patients with various types of colorectal lesions (advanced adenomas and stage II-IV colorectal cancers) and control (patients displaying no pathological findings) using duplex TaqMan reactions for both methods. ITGA6 and ITGA6A were chosen for this proof-of-concept study based on their relative medium and low abundance in stool samples, respectively, as established in a previous study. We found that the ddPCR and qPCR methods performed equally well in this TaqMan duplex assay for the detection of ITGA6 and ITGA6A transcripts in stools of patients with colorectal lesions. For ITGA6, receiver operating characteristic (ROC) curve analysis showed comparable areas under the curve of 0.91 (P < 0.0001) and 0.89-0.90 (P < 0.0001) for the prediction of advanced adenomas and colorectal cancers, respectively. ITGA6A, which was detected at very low levels in control patients, was found to be significantly elevated ...Continue Reading

References

Feb 13, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ying Jie YuIkuko Kato
Dec 25, 2008·Histochemistry and Cell Biology·Anders Bondo DydensborgJean-François Beaulieu
Jan 7, 2009·Gastroenterology Research and Practice·Yoshikatsu KogaYasuhiro Matsumura
Feb 5, 2009·Annals of Internal Medicine·Sabrina HundtHermann Brenner
Jun 10, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Tetsunari TakaiAkira Hishida
Jul 10, 2009·BMC Cancer·Anders Bondo DydensborgJean-François Beaulieu
May 25, 2011·World Journal of Gastrointestinal Pathophysiology·Jean-François Beaulieu
Sep 3, 2013·Nature Methods·Christopher M HindsonMuneesh Tewari
Oct 9, 2013·Clinical Chemistry·Jim F Huggett, Alexandra Whale
Mar 25, 2014·Annals of Internal Medicine·Jeffrey K LeeDouglas A Corley
Apr 25, 2014·Advanced Biomedical Research·Mohamadhasan TajadiniShaghayegh Haghjooy Javanmard
Jul 10, 2014·The New England Journal of Medicine·Thomas F ImperialeSteven H Itzkowitz
Jul 10, 2014·The New England Journal of Medicine·John H M Souverijn
Aug 12, 2014·Expert Review of Molecular Diagnostics·Helena ZecTza-Huei Wang
Aug 17, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Claudia RengucciDaniele Calistri
Oct 4, 2014·Diagnostic Microbiology and Infectious Disease·Ruth Hall SedlakKeith R Jerome
Oct 24, 2014·Clinical Chemistry·Jim F HuggettCarole A Foy
May 15, 2015·Nature·Cassandra Willyard
Jun 3, 2015·Gastroenterology·Douglas J Robertson, Thomas F Imperiale
Jul 5, 2015·BMC Genomics·Michael ReckUNKNOWN COMBACTE Consortium
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 12, 2016·Cellular Immunology·Jennifer StauberMark J Manary

❮ Previous
Next ❯

Citations

Nov 3, 2017·World Journal of Gastroenterology : WJG·Susana Olmedillas-LópezDamián García-Olmo
Sep 11, 2018·Cancer Biomarkers : Section a of Disease Markers·Lin ZhuHong Yu
May 6, 2021·International Journal of Molecular Sciences·Vincenza Ylenia CusenzaRaffaele Frazzi
Nov 14, 2021·Molecular Diagnosis & Therapy·Susana Olmedillas-LópezDamián García-Olmo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
biopsy
Assay
RNA assay

Software Mentioned

GraphPad
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.