Droplet formation and shrinking in aqueous two-phase systems using a membrane emulsification method

Biomicrofluidics
Hans Breisig, Matthias Wessling

Abstract

Using a membrane emulsification method based on porous hollow-fiber membranes in combination with an aqueous two-phase system (ATPS), we are able to produce "water-in-water" droplets with narrow-dispersed size distributions. The equilibrium phases of the aqueous two-phase system polyethylene glycol-dipotassium hydrogen phosphate are used for this purpose. The droplet diameter of a given fluid system is determined by the flow rates of the continuous and disperse phase as well as the hollow fiber dimensions. When diluting the disperse phase and thus moving the ATPS system out of equilibrium, the droplet size can be further reduced in comparison to the equilibrium case. Generally, droplets formed with this method have diameters 20%-60% larger than the inner hollow fiber diameter. The new strategy of diluting the disperse phase allows the production of droplet diameter below the inner diameter of the membrane.

References

Aug 15, 1991·Analytical Biochemistry·H WalterD E Brooks
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·H O JohanssonC A Haynes
Dec 21, 2006·Lab on a Chip·G MünchowK S Drese
Oct 13, 2007·Physical Review Letters·Pierre GuillotArmand Ajdari
Oct 13, 2007·Physical Review Letters·Andrew S UtadaDavid A Weitz
May 8, 2010·Journal of Chromatography. a·Young Hoon ChoiDo Hyun Kim
Sep 8, 2011·Lab on a Chip·Steffen Hardt, Thomas Hahn
Apr 27, 2012·Biomicrofluidics·Sam D GeschiereMichiel T Kreutzer
Jun 5, 2012·Biomicrofluidics·Ho Cheung ShumDavid A Weitz
Apr 26, 2013·Analytical Chemistry·Taisuke Kojima, Shuichi Takayama
Apr 30, 2013·Journal of Physics D: Applied Physics·J K NunesH A Stone
Jul 30, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Ehsan AtefiHossein Tavana

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Citations

Nov 22, 2019·Chemical Society Reviews·Youchuang Chao, Ho Cheung Shum
Aug 17, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Christian J LinnartzMatthias Wessling

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