Droplet Incubation and Splitting in Open Microfluidic Channels

Analytical Methods : Advancing Methods and Applications
Samuel B BerryAshleigh B Theberge

Abstract

Droplet-based microfluidics enables compartmentalization and controlled manipulation of small volumes. Open microfluidics provides increased accessibility, adaptability, and ease of manufacturing compared to closed microfluidic platforms. Here, we begin to build a toolbox for the emerging field of open channel droplet-based microfluidics, combining the ease of use associated with open microfluidic platforms with the benefits of compartmentalization afforded by droplet-based microfluidics. We develop fundamental microfluidic features to control droplets flowing in an immiscible carrier fluid within open microfluidic systems. Our systems use capillary flow to move droplets and carrier fluid through open channels and are easily fabricated through 3D printing, micromilling, or injection molding; further, droplet generation can be accomplished by simply pipetting an aqueous droplet into an empty open channel. We demonstrate on-chip incubation of multiple droplets within an open channel and subsequent transport (using an immiscible carrier phase) for downstream experimentation. We also present a method for tunable droplet splitting in open channels driven by capillary flow. Additional future applications of our toolbox for droplet ma...Continue Reading

References

Mar 6, 2004·Physical Review Letters·D R LinkH A Stone
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·P Concus, R Finn
Nov 7, 2006·Angewandte Chemie·Helen SongRustem F Ismagilov
Jul 27, 2007·Lab on a Chip·Luis M FidalgoWilhelm T S Huck
Jan 31, 2008·Lab on a Chip·Shia-Yen TehAbraham P Lee
Dec 25, 2008·Lab on a Chip·Hakim BoukellalSeth Fraden
May 7, 2009·Lab on a Chip·Lucas FrenzAndrew D Griffiths
Oct 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Adam R AbateDavid A Weitz
Nov 26, 2010·Applied and Environmental Microbiology·Hiroyasu OnakaTamotsu Furumai
Apr 25, 2012·Annual Review of Analytical Chemistry·Kihwan ChoiAaron R Wheeler
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Benjamin P CasavantDavid J Beebe
Apr 24, 2015·Lab on a Chip·David J GuckenbergerEdmond W K Young
Dec 9, 2015·Annual Review of Biomedical Engineering·Alphonsus H C NgAaron R Wheeler
Dec 15, 2015·Lab on a Chip·T E de GrootA B Theberge
Feb 5, 2016·Nature Communications·Layla J BarkalErwin Berthier
May 26, 2017·Chemical Reviews·Luoran ShangYuanjin Zhao
Jun 18, 2017·Medical Engineering & Physics·J BerthierE Berthier
Jan 9, 2018·Lab on a Chip·Ulri N LeeAshleigh B Theberge
Apr 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Jing J LeeErwin Berthier
May 23, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Robert K LadeLorraine F Francis
Dec 21, 2018·Analytical Chemistry·Dominik HaidasPetra S Dittrich
Jul 19, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Jing J LeeErwin Berthier

❮ Previous
Next ❯

Citations

Jan 7, 2020·Lab on a Chip·Xing HuangYun Jie Ruan
Jun 9, 2020·Biomicrofluidics·Linfeng XuKwang W Oh
Jan 24, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Prasenjit KabiSaptarshi Basu

❮ Previous
Next ❯

Methods Mentioned

BETA
chip

Software Mentioned

Prism
ImageJ
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

BioRxiv : the Preprint Server for Biology
Samuel B BerryAshleigh B Theberge
Langmuir : the ACS Journal of Surfaces and Colloids
Jing J LeeErwin Berthier
Chemical Reviews
Luoran ShangYuanjin Zhao
Methods in Molecular Biology
Sanjiv SharmaAnthony Cass
© 2022 Meta ULC. All rights reserved