Drosophila DNA polymerase zeta interacts with recombination repair protein 1, the Drosophila homologue of human abasic endonuclease 1

The Journal of Biological Chemistry
Ryo TakeuchiKengo Sakaguchi

Abstract

Abasic (AP) sites are a threat to cellular viability and genomic integrity, since they impede transcription and DNA replication. In mammalian cells, DNA polymerase (pol) beta plays an important role in the repair of AP sites. However, it is known that many organisms, including Drosophila melanogaster, do not have a pol beta homologue, and it is unclear how they repair AP sites. Here, we screened for DNA polymerases that interact with the Drosophila AP endonuclease 1 homologue, Rrp1 (recombination repair protein 1), and found that Drosophila pol zeta (Dmpol zeta), DmREV3 and DmREV7 bound to Rrp1 in a protein affinity column. Rrp1 directly interacted with DmREV7 in vitro and in vivo but not with DmREV3. These findings suggest that the DNA polymerase partner for Rrp1 is Dmpol zeta and that this interaction occurs through DmREV7. Interestingly, DmREV7 bound to the N-terminal region of Rrp1, which has no known protein homologue, suggesting that this binding is a species-specific event. Moreover, DmREV7 could stimulate the AP endonuclease activity of Rrp1, but not the 3'-exonuclease activity, and form a homomultimer. DmREV3 could not incorporate nucleotides at the 5'-incised tetrahydrofran sites but did show strand displacement activ...Continue Reading

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M SanderA Rich
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B DempleD S Chen
Nov 25, 1993·Nucleic Acids Research·R PrasadS H Wilson
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SzakmaryM Sander
Jun 14, 1996·Science·J R NelsonD C Hinkle
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·R A BennettB Demple
Nov 7, 1998·The Journal of Biological Chemistry·Y MasudaB Demple
Dec 16, 1998·The Journal of Biological Chemistry·B J ReardonM Sander
Dec 3, 1999·Science·T Lindahl, R D Wood
Oct 26, 2000·Current Biology : CB·J WittschiebenR D Wood
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K KajiwaraM Kimura
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M DiazN R Klinman
Aug 4, 2001·The Journal of Biological Chemistry·Y MurakumoM Takahashi
Oct 2, 2001·The Journal of Biological Chemistry·P M BurgersR Woodgate
Jan 2, 2003·DNA Repair·Christopher W Lawrence
Nov 26, 2003·Mutation Research·Grigory L DianovSarah L Allinson
Jun 4, 2004·The Biochemical Journal·Ryo TakeuchiKengo Sakaguchi
Jun 23, 2004·European Journal of Biochemistry·Yukinobu UchiyamaKengo Sakaguchi
Apr 15, 2005·PLoS Biology·Stepánka VanácováWalter Keller

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Citations

Dec 25, 2007·Cell Research·Gregory N GanRichard D Wood
Feb 4, 2009·Molecular and Cellular Biology·Carlo VascottoGianluca Tell
Aug 14, 2010·Cellular and Molecular Life Sciences : CMLS·Gianluca TellFranco Quadrifoglio
Jan 22, 2009·The FEBS Journal·Kengo SakaguchiKazuki Iwabata
Apr 30, 2016·Wiley Interdisciplinary Reviews. RNA·Jekaterina VohhodinaKienan I Savage
Jan 15, 2020·Fly·Steven J MarygoldMasamitsu Yamaguchi

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