Drosophila FGF cleavage is required for efficient intracellular sorting and intercellular dispersal

The Journal of Cell Biology
Alex SohrSougata Roy

Abstract

How morphogenetic signals are prepared for intercellular dispersal and signaling is fundamental to the understanding of tissue morphogenesis. We discovered an intracellular mechanism that prepares Drosophila melanogaster FGF Branchless (Bnl) for cytoneme-mediated intercellular dispersal during the development of the larval Air-Sac-Primordium (ASP). Wing-disc cells express Bnl as a proprotein that is cleaved by Furin1 in the Golgi. Truncated Bnl sorts asymmetrically to the basal surface, where it is received by cytonemes that extend from the recipient ASP cells. Uncleavable mutant Bnl has signaling activity but is mistargeted to the apical side, reducing its bioavailability. Since Bnl signaling levels feedback control cytoneme production in the ASP, the reduced availability of mutant Bnl on the source basal surface decreases ASP cytoneme numbers, leading to a reduced range of signal/signaling gradient and impaired ASP growth. Thus, enzymatic cleavage ensures polarized intracellular sorting and availability of Bnl to its signaling site, thereby determining its tissue-specific intercellular dispersal and signaling range.

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Citations

Jun 25, 2019·Physical Biology·Hyunjoong Kim, Paul C Bressloff
Jun 28, 2019·Frontiers in Molecular Neuroscience·Hermann Aberle
May 10, 2019·Development·Laura González-MéndezIsabel Guerrero
Aug 20, 2019·Bio-protocol·Alex SohrSougata Roy
Jul 4, 2020·Current Biology : CB·Vincent StepanikAngelike Stathopoulos

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Methods Mentioned

BETA
transgenic
PCR
gene
transfection

Software Mentioned

Fiji
Gibson Assembly

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