Drosophila GPI-mannosyltransferase 2 is required for GPI anchor attachment and surface expression of chaoptin.

Visual Neuroscience
Erica E RosenbaumNansi J Colley

Abstract

Glycosylphosphatidylinositol (GPI) anchors are critical for the membrane attachment of a wide variety of essential signaling and cell adhesion proteins. The GPI anchor is a complex glycolipid structure that utilizes glycosylphosphatidylinositol-mannosyltransferases (GPI-MTs) for the addition of three core mannose residues during its biosynthesis. Here, we demonstrate that Drosophila GPI-MT2 is required for the GPI-mediated membrane attachment of several GPI-anchored proteins, including the photoreceptor-specific cell adhesion molecule, chaoptin. Mutations in gpi-mt2 lead to defects in chaoptin trafficking to the plasma membrane in Drosophila photoreceptor cells. In gpi-mt2 mutants, loss of sufficient chaoptin in the membrane leads to microvillar instability, photoreceptor cell pathology, and retinal degeneration. Finally, using site-directed mutagenesis, we have identified key amino acids that are essential for GPI-MT2 function and cell viability in Drosophila. Our findings on GPI-MT2 provide a mechanistic link between GPI anchor biosynthesis and protein trafficking in Drosophila and shed light on a novel mechanism for inherited retinal degeneration.

References

Nov 1, 1971·Kybernetik·N Franceschini, K Kirschfeld
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N J ColleyC S Zuker
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S E MaxwellS Udenfriend
Mar 1, 1994·Molecular & General Genetics : MGG·Y KimuraI Yahara
Jun 29, 1999·Biological Chemistry·A TiedeR E Schmidt
Sep 15, 2001·Nature·R C Hardie, P Raghu
Aug 30, 2002·Molecular Biology and Evolution·Rafael OriolPatrice Codogno
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Frances J Sharom, Marty T Lehto
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Domna Karagogeos
Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kisaburo NagamuneTaroh Kinoshita
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joanna M CordyAnthony J Turner
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Christina ZeitzWolfgang Berger
Dec 30, 2004·The Journal of Biological Chemistry·Ji Young KangTaroh Kinoshita
May 21, 2005·Human Molecular Genetics·Elizabeth O'ConnorDorothy Trump
Jun 13, 2006·Nature Medicine·Antonio M AlmeidaAnastasios Karadimitris
May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
Oct 5, 2007·Biochimica Et Biophysica Acta·Morihisa Fujita, Yoshifumi Jigami
Apr 9, 2008·Trends in Neurosciences·Samuel DavidRubèn López-Vales
Jun 19, 2008·Biochemistry·Margot G Paulick, Carolyn R Bertozzi
Jul 19, 2008·Journal of Biochemistry·Taroh KinoshitaYusuke Maeda
Oct 31, 2008·American Journal of Physiology. Renal Physiology·Olga VaginGeorge Sachs
Jun 23, 2009·Acta Tropica·Romanico B G Arrighi, Ingrid Faye
Jul 23, 2009·Frontiers in Cellular Neuroscience·Ben Katz, Baruch Minke
Oct 20, 2009·Cell·King-Wai Yau, Roger C Hardie
Feb 10, 2010·Chembiochem : a European Journal of Chemical Biology·Yug Varma, Tamara Hendrickson
Feb 11, 2010·Current Biology : CB·Gordon L FainSimon B Laughlin
Apr 29, 2010·Current Atherosclerosis Reports·Abdul HakeemMehmet Cilingiroglu
Aug 7, 2010·Investigative Ophthalmology & Visual Science·Kelly N MaRajendra Kumar-Singh
Oct 12, 2010·Journal of Biomedicine & Biotechnology·Chary López-PedreraMaria José Cuadrado

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.