Drosophila immunity: a comparative analysis of the Rel proteins dorsal and Dif in the induction of the genes encoding diptericin and cecropin

Nucleic Acids Research
I GrossJ A Hoffmann

Abstract

In Drosophila, bacterial challenge induces the rapid transcription of several genes encoding potent antibacterial peptides. The upstream sequences of the diptericin and cecropin Al genes, which have been investigated in detail, contain two, respectively one sequence element homologous to the binding site of the mammalian nuclear factor kappaB. These elements have been shown to be mandatory for immune-induced transcription of both genes. Functional studies have shown that these kappaB-related elements can be the target for the Drosophila Rel proteins dorsal and Dif. Here we present a comparative analysis of the transactivating capacities of these proteins on reporter genes fused to either the diptericin or the cecropin kappaB-related motifs. We conclude from our results: (i) the kappaB motifs of the diptericin and cecropin genes are not functionally equivalent; (ii) the dorsal and Dif proteins have distinct DNA-binding characteristics; (iii) dorsal and Dif can heterodimerize in vitro; (iv) mutants containing no copies of dorsal and a single copy of Dif retain their full capacity to express the diptericin and cecropin genes in response to challenge.

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·C SamakovlisD Hultmark
Aug 15, 1991·European Journal of Biochemistry·B R Williams
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1995·Advances in Immunology·E B Kopp, S Ghosh
Feb 1, 1995·Current Opinion in Immunology·J A Hoffmann
Oct 1, 1994·Current Opinion in Genetics & Development·Y T Ip, M Levine
Aug 1, 1993·Current Opinion in Genetics & Development·R Steward, S Govind
Jul 20, 1993·Journal of Molecular Biology·Y EngströmI Faye
Jan 1, 1993·International Review of Cytology·M GrilliM J Lenardo

❮ Previous
Next ❯

Citations

Jun 16, 2001·Journal of Cellular Biochemistry·W D Cornwell, R B Kirkpatrick
Jul 30, 1999·Developmental and Comparative Immunology·Y Engström
Apr 18, 2000·Gene·M S DushayE D Eldon
May 1, 1997·Insect Biochemistry and Molecular Biology·W L ChoC C Chen
May 5, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L Du Pasquier
May 19, 2000·Cytokine & Growth Factor Reviews·T K MeansM J Fenton
Sep 17, 2002·Insect Molecular Biology·K Roxström-LindquistI Faye
Dec 4, 2003·Annual Review of Entomology·Jeremy A Kroemer, Bruce A Webb
Jan 5, 2007·Annual Review of Immunology·Bruno Lemaitre, Jules Hoffmann
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·P IrvingC Hetru
Sep 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiangzhong ZhengAmita Sehgal
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
May 29, 2007·Trends in Genetics : TIG·Hanna Uvell, Ylva Engström
Jun 24, 2004·Journal of Cellular Biochemistry·Raul BettencourtY Tony Ip
Jul 31, 2007·The EMBO Journal·Matthew S BusseSteven A Wasserman
Jan 1, 2014·Journal of Cellular Physiology·Puja Verma, Madhu G Tapadia
Feb 27, 2016·Genes, Brain, and Behavior·B R TroutwineN S Atkinson
Apr 10, 2012·Insect Biochemistry and Molecular Biology·Hiromitsu TanakaMinoru Yamakawa
Oct 3, 2012·PLoS Pathogens·Ezequiel WexselblattSigal Ben-Yehuda
Mar 13, 2015·PLoS Genetics·Robert L UncklessBrian P Lazzaro
Dec 19, 2016·Gene·Hiromitsu Tanaka, Aki Sagisaka
Dec 15, 2016·Frontiers in Physiology·Alex P ArpKirsten S Pelz-Stelinski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.