Drosophila immunity. A sequence homologous to mammalian interferon consensus response element enhances the activity of the diptericin promoter

Nucleic Acids Research
P GeorgelJ A Hoffmann

Abstract

Bacterial challenge of larvae or adults of Drosophila induces the rapid transcription of several genes encoding antibacterial peptides with a large spectrum of activity. One of these peptides, the 82-residue anti-gram negative diptericin, is encoded by a single intronless gene and we are investigating the control of expression of this gene. Previous studies using both transgenic experiments and footprint analysis have highlighted the role in the induction of this gene of a 30 nucleotide region which contains three partially overlapping motifs with sequence homology to mammalian NF-kappa B and NF-IL6 response elements and to the GAAANN sequence present in the interferon consensus response elements of some mammalian interferon-induced genes. We now show that the latter sequence binds in immune responsive tissues (fat body, blood cells) of Drosophila a approximately 45 kDa polypeptide which cross-reacts with a polyserum directed against mammalian interferon Regulatory Factor-I. Using a transfection assay of Drosophila tumorous blood cells, we show that the GAAANN sequence positively regulates the activity of the diptericin promoter. We propose that this motif cooperatively interacts with the other response elements in the regulati...Continue Reading

References

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Citations

Dec 12, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M MeisterJ A Hoffmann
Jun 16, 2001·Journal of Cellular Biochemistry·W D Cornwell, R B Kirkpatrick
Jul 30, 1999·Developmental and Comparative Immunology·Y Engström
Feb 1, 1996·Current Opinion in Immunology·J A HoffmannC Hetru
Dec 6, 2002·Nucleic Acids Research·Mi-Sun HwangMi-Ae Yoo
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Jan 28, 1998·Annual Review of Entomology·L G Harshman, A A James
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·A Bernal, D A Kimbrell
Oct 24, 2009·Veterinary Immunology and Immunopathology·Alexander ReblHans-Martin Seyfert
Dec 18, 2007·Fish & Shellfish Immunology·Sirinit TharntadaAnchalee Tassanakajon
Mar 17, 2011·The EMBO Journal·Kwang-Zin Lee, Dominique Ferrandon
Aug 11, 2020·Frontiers in Immunology·Srishti Arora, Petros Ligoxygakis
Nov 11, 1996·Biochimica Et Biophysica Acta·G C Paesen, P A Nuttall
Aug 1, 1997·Trends in Cell Biology·J A Hergannan, J V Rechhart

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