PMID: 8608940Apr 15, 1996Paper

Drosophila IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivo

Genes & Development
E L Beall, D C Rio

Abstract

The P family of transposable elements in Drosophila transpose by a cut-and-paste mechanism involving double-strand gap repair. We report here that a Drosophila mutagen-sensitive mutant, mus3O9, contains a mutation in IRBP (inverted repeat binding protein), the Drosophila homolog of the mammalian Ku p70 gene. We show that the repair of double-strand DNA breaks after P-element excision is severely reduced in mus3O9 mutants using an in vivo assay for P-element transposase activity. In addition, excision products recovered from mus3O9 mutant embryos by use of a plasmid-based P-element mobility assay contain large deletions, suggesting that IRBP is involved in the repair of double-strand DNA breaks. Our findings provide the first demonstration that a mutation in the IRBP gene affects double-strand DNA break repair and suggest that DNA repair functions are conserved between Drosophila and mammals.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W R Engels
Sep 1, 1979·Mutation Research·G BronzettiH V Malling
Mar 1, 1992·Molecular & General Genetics : MGG·E Takasu-IshikawaY Hotta
Mar 1, 1991·Molecular & General Genetics : MGG·D A O'BrochtaA M Handler
Oct 25, 1991·Nucleic Acids Research·S Paillard, F Strauss
May 1, 1989·Genes & Development·M C MullinsG M Rubin
Oct 20, 1989·Cell·H W Benjamin, N Kleckner
Mar 20, 1988·Journal of Molecular Biology·D C RioG M Rubin
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D C Rio, G M Rubin
May 26, 1987·Nucleic Acids Research·R KlemenzW J Gehring
Aug 1, 1984·Cell·R E Karess, G M Rubin
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·G S BrushT J Kelly
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·E L BeallD C Rio
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·N J FinnieS P Jackson
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·N V BoubnovE A Hendrickson
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·W K Rathmell, G Chu
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H MessierA Fotedar
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A O'brochta, A M Handler

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Citations

Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D H Lankenau, G B Gloor
Oct 27, 1997·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·M Z Zdzienicka
Jun 17, 2000·Mutation Research·J DucauC de La Roche Saint-André
Sep 1, 2011·Nature Communications·Uffe HellstenDaniel S Rokhsar
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·F LiangM Jasin
Jul 27, 2001·Nucleic Acids Research·H PospiechJ E Syväoja
Aug 15, 1997·Genes & Development·E L Beall, D C Rio
Jan 26, 2013·Science·Sharmistha MajumdarDonald C Rio
Jan 1, 1997·Annual Review of Biochemistry·N L Craig
Apr 17, 2007·PLoS Genetics·Dena M Johnson-SchlitzWilliam R Engels
Jan 24, 2014·PLoS Genetics·Mireille BétermierBernard S Lopez
Mar 23, 2005·Genetics·Larisa MelnikovaPavel Georgiev
Feb 3, 2006·Genetics·Kelly BeumerDana Carroll
Sep 5, 2006·Genetics·Alexander EmelyanovSerguei Parinov
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jan LarssonMaria Mäkitalo
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mitch McVeyJeff J Sekelsky
Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jody L PlankTao-Shih Hsieh
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bosun MinDonald C Rio
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Feschotte, S R Wessler
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·G Barnes, D Rio
Jul 26, 2000·The Journal of Cell Biology·J J SekelskyK C Burtis
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Jul 29, 2004·DNA Repair·Matthew D WeitzmanCaroline E Lilley
May 18, 2016·Chemical Reviews·Alison B Hickman, Fred Dyda
Sep 24, 2016·International Journal of Radiation Biology·Elena Yushkova, Vladimir Zainullin
Nov 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Malik Joseph FrancisDonald C Rio

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