PMID: 6404246Feb 1, 1983Paper

Drosophila lactate dehydrogenase: developmental aspects

Biochemical Genetics
S N AlahiotisM Pelecanos

Abstract

Partially purified lactate dehydrogenase (LDH) from third-instar larvae displays two bands (one major and one minor) on polyacrylamide gels. Analogous preparations from pupae and adults exhibit three LDH-staining bands (one major and two minor) in a similar pattern. The migration of the major band is similar for larvae, pupae, and adults, while the two minor LDH bands of pupae and adults migrate more slowly than the minor larval band. It has been shown that larval LDH incubated with beta-nicotinamide adenine dinucleotide exhibits two additional minor bands with an electrophoretic mobility similar to that of the minor bands of both pupae and adults. The intensity of the minor larval LDH band (exhibited also by untreated preparations) is drastically reduced. This fact indicates that the life-cycle stage-dependent LDH isozymic distribution is possibly due to a posttranslational effect(s). Highly purified LDH from larvae, pupae, or adults, obtained by an affinity chromatography procedure, displays just one dispersed band, located in the area between the band 5 and the band 6 exhibited by crude extract preparations. These data, in combination with the lack of difference in catalytic properties among enzymes from larvae, pupae, and a...Continue Reading

References

Jun 1, 1976·Biochemical Genetics·T Agatsuma, T Takeuchi
Mar 1, 1972·Archives of Biochemistry and Biophysics·J A Knopp, K B Jacobson
Nov 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P W Pappas, G E Rodrick
May 1, 1970·Journal of Insect Physiology·M C Rechsteiner
Dec 1, 1982·Biochemical Genetics·A Onoufriou, S N Alahiotis

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Citations

Jan 1, 1993·Progress in Biophysics and Molecular Biology·L A Fothergill-Gilmore, P A Michels
Oct 3, 2008·BMC Evolutionary Biology·Melania E CristescuTeresa J Crease

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