PMID: 9183014May 1, 1997Paper

Drosophila laminin binds to mammalian nidogen and to heparan sulfate proteoglycan

European Journal of Biochemistry
U MayerR Timpl

Abstract

A Drosophila laminin that has the chain composition alpha5 beta1 gamma1, relative to mammalian laminins, bound human and mouse nidogen almost as strongly as mouse laminin-1 (alpha1 beta1 gamma1) in solid-phase assays, and had only a fourfold lower affinity in a radioligand competition test. This is due to a short, highly conserved sequence that occurs in both laminin gamma1 chains and which binds nidogen. When the single conservative amino acid difference between the two sequences (Tyr-->His) was introduced into the mouse laminin binding module gamma1 III4 it failed to cause any change of binding. A high affinity between Drosophila laminin and mouse nidogen resulted in the formation of a stable complex in solution. Drosophila laminin also bound to the mouse heparan sulfate proteoglycan perlecan and the formation of this complex was inhibited by heparin, but not by chondroitin sulfate. In addition, a weaker connection between the core protein of mouse perlecan and Drosophila laminin can be mediated through nidogen. Elastase and other proteases degraded Drosophila laminin to a restricted number of larger fragments (40-300 kDa), almost all of which were bound to a heparin affinity column. Three fragments could be displaced at low ...Continue Reading

References

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Citations

Apr 23, 2002·Journal of Cellular Biochemistry·Karin AlvarezEnrique Brandan
May 20, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M S Parker, D D Ourth
May 29, 2000·European Journal of Biochemistry·M V FriedrichS Baumgartner
Apr 12, 2011·Tissue Engineering. Part B, Reviews·Jennifer Y C ChengLaura Poole-Warren
Dec 12, 2012·Journal of Molecular Microbiology and Biotechnology·Angelika RothRegine Hakenbeck
Sep 10, 2002·Developmental Biology·Jennifer BonnerTimothy O'Connor
Jun 5, 2003·Nature Reviews. Cancer·Raghu Kalluri
Jul 27, 2004·Archives of Insect Biochemistry and Physiology·Yasumitsu TakagiSetsuo Hirohashi

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