Drosophila MAGI interacts with RASSF8 to regulate E-Cadherin-based adherens junctions in the developing eye

Development
Sophie ZaessingerAlexandre Djiane

Abstract

Morphogenesis is crucial during development to generate organs and tissues of the correct size and shape. During Drosophila late eye development, interommatidial cells (IOCs) rearrange to generate the highly organized pupal lattice, in which hexagonal ommatidial units pack tightly. This process involves the fine regulation of adherens junctions (AJs) and of adhesive E-Cadherin (E-Cad) complexes. Localized accumulation of Bazooka (Baz), the Drosophila PAR3 homolog, has emerged as a critical step to specify where new E-Cad complexes should be deposited during junction remodeling. However, the mechanisms controlling the correct localization of Baz are still only partly understood. We show here that Drosophila Magi, the sole fly homolog of the mammalian MAGI scaffolds, is an upstream regulator of E-Cad-based AJs during cell rearrangements, and that Magi mutant IOCs fail to reach their correct position. We uncover a direct physical interaction between Magi and the Ras association domain protein RASSF8 through a WW domain-PPxY motif binding, and show that apical Magi recruits the RASSF8-ASPP complex during AJ remodeling in IOCs. We further show that this Magi complex is required for the cortical recruitment of Baz and of the E-Cad-as...Continue Reading

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Citations

May 7, 2016·Cellular and Molecular Life Sciences : CMLS·Peter Coopman, Alexandre Djiane
May 20, 2017·Nature Communications·Africa CoutoMarios Georgiou
May 20, 2018·Physical Review. E·Keiji OkumuraAtsuko Takamatsu
Sep 28, 2017·Nature Communications·Yavuz KulaberogluAlexander Hergovich
Feb 10, 2021·G3 : Genes - Genomes - Genetics·Miles W DeAngelisRuth I Johnson

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