PMID: 9445383Mar 7, 1998Paper

Drosophila melanogaster alcohol dehydrogenase: mechanism of aldehyde oxidation and dismutation

The Biochemical Journal
J O Winberg, J S McKinley-McKee

Abstract

Drosophila alcohol dehydrogenase (Adh) catalyses the oxidation of both alcohols and aldehydes. In the latter case, the oxidation is followed by a reduction of the aldehyde, i.e. a dismutation reaction. At high pH, dismutation is accompanied by a small release of NADH, which is not observed at neutral pH. Previously it has been emphasized that kinetic coefficients obtained by measuring the increase in A340, i.e. the release of NADH at high pH is not a direct measure of the aldehyde oxidation reaction and these values cannot be compared with those for alcohol dehydrogenation. In this article we demonstrate that this is not entirely true, and that the coefficients phiB and phiAB, where B is the aldehyde and A is NAD+, are the same for a dismutation reaction and a simple aldehyde dehydrogenase reaction. Thus the substrate specificity of the aldehyde oxidation reaction can be determined by simply measuring the NADH release. The coefficients for oxidation and dehydrogenation reactions (phi0d and phiAd respectively) are complex and involve the constants for the dismutation reaction. However, dead-end inhibitors can be used to determine the quantitative contribution of the kinetic constants for the aldehyde oxidation and reduction path...Continue Reading

Citations

Feb 19, 2011·Chemico-biological Interactions·Aída Hernández-TobíasHéctor Riveros-Rosas
Sep 14, 2010·The International Journal of Biochemistry & Cell Biology·Kumari AlkaGary T M Henehan
Nov 6, 2007·Biophysical Journal·Osman A B S M GaniIngebrigt Sylte
Apr 18, 2001·Chemico-biological Interactions·J BenachR Gonzàlez-Duarte
Nov 7, 2009·The Biochemical Journal·Patricia FerreiraAngel T Martínez
Sep 3, 2010·Journal of Molecular Evolution·Jianming ZhangAntony M Dean
Aug 7, 2003·Applied and Environmental Microbiology·Jung-Kul LeeHyung-Hwan Hyun
Apr 21, 1999·Biochimica Et Biophysica Acta·M Kaaber BrendskagJ O Winberg

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