Drosophila melanogaster as a model host for the Burkholderia cepacia complex.

PloS One
Josée Castonguay-VanierEric Déziel

Abstract

Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms. The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obta...Continue Reading

References

May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
May 30, 1998·The Journal of Biological Chemistry·E NicolasB Lemaitre
Jan 28, 1999·Journal of Bacteriology·S LewenzaP A Sokol
Dec 22, 1999·Infection and Immunity·D W Martin, C D Mohr
Feb 3, 2000·Journal of Clinical Microbiology·E MahenthiralingamP Vandamme
Mar 25, 2000·Science·M D AdamsJ C Venter
Apr 14, 2000·American Journal of Respiratory and Critical Care Medicine·S D AaronN E Macdonald
Jul 19, 2000·Current Biology : CB·M Elrod-EricksonD Schneider
Feb 7, 2001·Journal of Bacteriology·D A D'ArgenioC Manoil
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·S A ChuganiE P Greenberg
Apr 5, 2001·Trends in Immunology·P G FallonT Rich
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·T CoenyeP Vandamme
Sep 28, 2001·Journal of Clinical Microbiology·T CoenyeJ J LiPuma
Oct 6, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E MahenthiralingamD P Speert
Jan 29, 2002·Nature Immunology·Jules A Hoffmann, Jean-Marc Reichhart
Dec 12, 2002·Methods in Enzymology·Regina L BaldiniLaurence G Rahme
Mar 22, 2003·Research in Microbiology·Peter VandammeJohn R W Govan
May 23, 2003·Infection and Immunity·Marc S DionneDavid S Schneider
Jun 7, 2003·Journal of Clinical Microbiology·Tom CoenyeEshwar Mahenthiralingam
Aug 9, 2003·Microbiology·C R CorbettP A Sokol
Apr 13, 2004·Eukaryotic Cell·Yiorgos ApidianakisEleftherios Mylonakis
May 4, 2004·Trends in Microbiology·Nicolas VodovarFrédéric Boccard
Jun 24, 2004·Infection and Immunity·Tracey A HuntMiguel A Valvano
Aug 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ted BrummelSeymour Benzer
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yiorgos ApidianakisLaurence G Rahme
Jun 24, 2005·Infection and Immunity·Eleftherios Mylonakis, Alejandro Aballay
Jul 26, 2005·FEMS Microbiology Letters·Thomas R LawsRichard W Titball
Jul 27, 2005·FEMS Microbiology Letters·Silvia T CardonaMiguel A Valvano
May 11, 2006·Trends in Microbiology·Luigi ChiariniPaolo Visca
Jul 14, 2007·Cellular Microbiology·Silvia A SousaGerd Döring
Oct 3, 2007·Antimicrobial Agents and Chemotherapy·David J WeberEmily E Sickbert-Bennett

❮ Previous
Next ❯

Citations

Dec 13, 2012·Future Microbiology·Sujatha Subramoni, Pamela A Sokol
Dec 5, 2012·PloS One·Martina Pilátová, Marc S Dionne
Mar 1, 2012·Journal of Oral Microbiology·Christina O IgboinEugene J Leys
Jan 11, 2016·Current Biology : CB·Victoria W AllenMimi M Shirasu-Hiza
Feb 6, 2013·MicrobiologyOpen·Annelise ChapalainEric Déziel
Apr 20, 2016·Vaccines·Gonzalo A PradenasAlfredo G Torres
Feb 24, 2015·Frontiers in Microbiology·Yamilé López HernándezIsidre Gibert
Apr 27, 2012·FEMS Immunology and Medical Microbiology·Sarah Edwards, Birthe V Kjellerup
Mar 28, 2018·BMC Research Notes·Cynthia Gagné-ThiviergeSteve J Charette
Jan 23, 2019·ILAR Journal·Lon V KendallJessica D Ayers
Mar 21, 2021·Applied and Environmental Microbiology·Samuel HendryDmitri Mavrodi
Nov 11, 2021·Applied Microbiology and Biotechnology·Viola Camilla ScoffoneSilvia Buroni

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPrism

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
R Seabra, N Bhogal
Advances in Applied Microbiology
Yvonne Sun, Mary X D O'Riordan
© 2021 Meta ULC. All rights reserved