Drosophila melanogaster p24 trafficking proteins have vital roles in development and reproduction

Mechanisms of Development
Sehresh SaleemGinger E Carney

Abstract

p24 proteins comprise a family of type-I transmembrane proteins of ~24kD that are present in yeast and plants as well as metazoans ranging from Drosophila to humans. These proteins are most commonly localized to the endoplasmic reticulum (ER)-Golgi interface and are incorporated in anterograde and retrograde transport vesicles. Little is known about how disruption of p24 signaling affects individual tissue function or whole animals. Drosophila melanogaster express nine p24 genes, grouped into four subfamilies. Based upon our mRNA and protein expression data, Drosophila p24 family members are expressed in a variety of tissues. To identify functions for particular Drosophila p24 proteins, we used RNA interference (RNAi) to reduce p24 expression. Ubiquitous reduction of most p24 genes resulted in complete or partial lethality during development. We found that reducing p24 levels in adults caused defects in female fecundity (egg laying) and also reduced male fertility. We attributed reduced female fecundity to decreased neural p24 expression. These results provide the first genetic analysis of all p24 family members in a multicellular animal and indicate vital roles for Drosophila p24s in development and reproduction, implicating n...Continue Reading

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Citations

Aug 21, 2013·Nature Reviews. Genetics·Sidi ChenManyuan Long
Aug 27, 2013·PloS One·Gunnar NewquistThomas Kidd
Aug 1, 2015·Protoplasma·Noelia Pastor-CantizanoFernando Aniento
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Jun 15, 2017·Mechanisms of Development·Lathiena ManningMichelle Starz-Gaiano
May 18, 2021·Parasitology International·Coh-Ichi Nihei, Masayuki Nakanishi
Oct 12, 2021·Biochimie·Danielly C A M MotaLuis F S Mendes

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