Drosophila melanogaster positive transcriptional elongation factors regulate metabolic and sex-biased expression in adults

BMC Genomics
Haiwang YangBrian Oliver

Abstract

Transcriptional elongation is a generic function, but is also regulated to allow rapid transcription responses. Following relatively long initiation and promoter clearance, RNA polymerase II can pause and then rapidly elongate following recruitment of positive elongation factors. Multiple elongation complexes exist, but the role of specific components in adult Drosophila is underexplored. We conducted RNA-seq experiments to analyze the effect of RNAi knockdown of Suppressor of Triplolethal and lilliputian. We similarly analyzed the effect of expressing a dominant negative Cyclin-dependent kinase 9 allele. We observed that almost half of the genes expressed in adults showed reduced expression, supporting a broad role for the three tested genes in steady-state transcript abundance. Expression profiles following lilliputian and Suppressor of Triplolethal RNAi were nearly identical raising the possibility that they are obligatory co-factors. Genes showing reduced expression due to these RNAi treatments were short and enriched for genes encoding metabolic or enzymatic functions. The dominant-negative Cyclin-dependent kinase 9 profiles showed both overlapping and specific differential expression, suggesting involvement in multiple co...Continue Reading

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Datasets Mentioned

BETA
GSE77492

Methods Mentioned

BETA
RNA-seq
transgenic
dissection

Software Mentioned

HTSeq
bedtools
DAVID
DESeq2
R
FlyAtlas
pheatmap
STAR

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