PMID: 6412136Aug 1, 1983Paper

Drosophila mutations at the mei-9 and mus(2)201 loci which block excision of thymine dimers also block induction of unscheduled DNA synthesis by methyl methanesulfonate, ethyl methanesulfonate, N-methyl-N-nitrosourea, UV light and X-rays

Mutation Research
R L DusenberyP D Smith

Abstract

The mei-9 and mus(2)201 mutants of Drosophila melanogaster were identified as mutagen-sensitive mutants on the basis of larval hypersensitivity to methyl methanesulfonate and characterized as excision repair-deficient on the basis of a greatly reduced capacity to excise thymine dimers from cellular DNA. The high degree of larval cytotoxicity observed with a variety of other chemical and physical agents indicated that these mutants may be unable to excise other important classes of DNA adducts. We have measured the ability of the single mutants and the double mutant combination mei-9;mus(2)201 to perform the resynthesis step in excision repair by means of an autoradiographic analysis of unscheduled DNA synthesis (UDS) induced in a mixed population of primary cells in culture. The 3 strains exhibit no detectable UDS activity in response to applied doses of 1.5-6.0 mM methyl methanesulfonate, 1.0-4.5 mM N-methyl-N-nitrosourea or 10-40 J/m2 254-nm UV light, dose ranges in which control cells exhibit a strong dose-dependent UDS response. The mei-9 and mei-9;mus(2)201 mutants also have no detectable UDS response to X-ray doses of 300-1800 rad, whereas the mus(2)201 mutant exhibits a reduced, but dose-dependent, response over this ran...Continue Reading

References

Aug 1, 1978·Photochemistry and Photobiology·K C Smith
Aug 1, 1974·Mutation Research·B Cox, J Game
Jan 1, 1982·Annual Review of Biochemistry·T Lindahl
Jan 1, 1981·Molecular & General Genetics : MGG·T C Brown, J B Boyd
Jan 1, 1982·Molecular & General Genetics : MGG·J B Boyd, K E Shaw
Jan 1, 1982·Advances in Genetics·C W Lawrence

❮ Previous
Next ❯

Citations

Aug 1, 1995·Mutation Research·E W Vogel, A T Natarajan
Mar 13, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Manish MishraD Kar Chowdhuri
Apr 16, 1998·Mutation Research·P G de Buendía
Mar 1, 1993·Mutation Research·H HakovirtaJ Lähdetie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.