Drosophila synaptotagmin I null mutants show severe alterations in vesicle populations but calcium-binding motif mutants do not

The Journal of Comparative Neurology
Carin A LoewenNoreen E Reist

Abstract

Synaptotagmin I is a synaptic vesicle protein postulated to mediate vesicle docking, vesicle recycling, and the Ca(2+) sensing required to trigger vesicle fusion. Analysis of synaptotagmin I knockouts (sytI(NULL) mutants) in both Drosophila and mice led to these hypotheses. Although much research on the mechanisms of synaptic transmission in Drosophila is performed at the third instar neuromuscular junction, the ultrastructure of this synapse has never been analyzed in sytI(NULL) mutants. Here we report severe synaptic vesicle depletion, an accumulation of large vesicles, and decreased vesicle docking at sytI(NULL) third instar neuromuscular junctions. Mutations in synaptotagmin I's C(2)B Ca(2+)-binding motif nearly abolish synaptic transmission and decrease the apparent Ca(2+) affinity of neurotransmitter release. Although this result is consistent with disruption of the Ca(2+) sensor, synaptic vesicle depletion and/or redistribution away from the site of Ca(2+) influx could produce a similar phenotype. To address this question, we examined vesicle distributions at neuromuscular junctions from third instar C(2)B Ca(2+)-binding motif mutants and transgenic wild-type controls. The number of docked vesicles and the overall number...Continue Reading

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Citations

Feb 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brie E PaddockNoreen E Reist
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amelia R StriegelNoreen E Reist
Aug 6, 2003·Trends in Neurosciences·Tong Wey Koh, Hugo J Bellen
Feb 24, 2012·PLoS Computational Biology·Denis MenshykauDagmar Iber
Apr 10, 2009·Genesis : the Journal of Genetics and Development·Kimberly E MaceNoreen E Reist
Jun 19, 2012·The EMBO Journal·Jörg MalsamThomas H Söllner
Feb 15, 2017·Molecular Neurodegeneration·Katarzyna Marta ZoltowskaOksana Berezovska

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