Drosophila Vps4 promotes Epidermal growth factor receptor signaling independently of its role in receptor degradation

Development
Kevin LegentJessica E Treisman

Abstract

Endocytic trafficking of signaling receptors is an important mechanism for limiting signal duration. Components of the Endosomal Sorting Complexes Required for Transport (ESCRT), which target ubiquitylated receptors to intra-lumenal vesicles (ILVs) of multivesicular bodies, are thought to terminate signaling by the epidermal growth factor receptor (EGFR) and direct it for lysosomal degradation. In a genetic screen for mutations that affect Drosophila eye development, we identified an allele of Vacuolar protein sorting 4 (Vps4), which encodes an AAA ATPase that interacts with the ESCRT-III complex to drive the final step of ILV formation. Photoreceptors are largely absent from Vps4 mutant clones in the eye disc, and even when cell death is genetically prevented, the mutant R8 photoreceptors that develop fail to recruit surrounding cells to differentiate as R1-R7 photoreceptors. This recruitment requires EGFR signaling, suggesting that loss of Vps4 disrupts the EGFR pathway. In imaginal disc cells mutant for Vps4, EGFR and other receptors accumulate in endosomes and EGFR target genes are not expressed; epistasis experiments place the function of Vps4 at the level of the receptor. Surprisingly, Vps4 is required for EGFR signaling ...Continue Reading

References

Jan 19, 1999·Current Topics in Developmental Biology·L A Nilson, T Schüpbach
Mar 6, 2002·The Journal of Biological Chemistry·Ekaterini PanopoulouTheodore Fotsis
Jul 23, 2002·Nature Genetics·Daimark Bennett, Luke Alphey
May 14, 2003·The Journal of Cell Biology·Tanja WucherpfennigMarcos González-Gaitán
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·R Grace ZhaiHugo J Bellen
Jul 21, 2004·Current Opinion in Cell Biology·Marta MiaczynskaMarino Zerial
Oct 14, 2004·Traffic·Aude de GassartMichel Vidal
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sara SigismundSimona Polo
Sep 30, 2005·The EMBO Journal·Anna ScottWesley I Sundquist
Nov 1, 2005·Developmental Cell·Barry J ThompsonStephen M Cohen
Nov 1, 2005·Nature Cell Biology·Han Lu, David Bilder

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Citations

Mar 5, 2016·Cellular and Molecular Life Sciences : CMLS·Marianne Malartre
Nov 2, 2016·Autophagy·Anindita MukherjeeAndreas Jenny
May 17, 2018·Essays in Biochemistry·Ana-Citlali GradillaIsabel Guerrero

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