Drp1 knockdown induces severe muscle atrophy and remodelling, mitochondrial dysfunction, autophagy impairment and denervation.

The Journal of Physiology
Maude DulacGilles Gouspillou

Abstract

The maintenance of optimal mitochondrial content and function is critical for muscle health. Mitochondrial dynamics play key roles in mitochondrial quality control; however, the exact role that mitochondrial fission plays in skeletal muscle health remains unclear. Here we report knocking down Drp1 (a protein regulating mitochondrial fission) for 4 months in adult mouse skeletal muscle resulted in severe muscle atrophy (40-50%). Drp1 knockdown also led to a reduction in ADP-stimulated respiration, an increase in markers of impaired autophagy and increased muscle regeneration, denervation, fibrosis and oxidative stress. Our data indicate that Drp1 is crucial for the maintenance of normal mitochondrial function and that Drp1 depletion severely impairs muscle health. Mitochondria play central roles in skeletal muscle physiology, including energy supply, regulation of energy-sensitive signalling pathways, reactive oxygen species production/signalling, calcium homeostasis and the regulation of apoptosis. The maintenance of optimal mitochondrial content and function is therefore critical for muscle cells. Mitochondria are now well known as highly dynamic organelles, able to change their morphology through fusion and fission processes....Continue Reading

References

Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J Covault, J R Sanes
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
May 18, 2006·Annual Review of Cell and Developmental Biology·David C Chan
Sep 9, 2006·The American Journal of Clinical Nutrition·Robert R Wolfe
Mar 17, 2007·Annual Review of Biochemistry·Suzanne HoppinsJodi Nunnari
Dec 7, 2007·Cell Metabolism·Cristina MammucariMarco Sandri
Apr 20, 2010·The EMBO Journal·Vanina RomanelloMarco Sandri
Jan 15, 2011·Journal of Biochemistry·Hidenori Otera, Katsuyoshi Mihara
Jun 29, 2011·The Journal of Physiology·Martin PicardRussell T Hepple
Dec 12, 2012·American Journal of Physiology. Cell Physiology·Michael F O'LearyDavid A Hood
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jun 9, 2015·Oncotarget·Jean-Philippe Leduc-GaudetGilles Gouspillou
Oct 7, 2015·The Journal of Physiology·Russell T Hepple, Charles L Rice
Dec 3, 2015·Current Protocols in Mouse Biology·Jorming Goh, Warren Ladiges
Jun 16, 2016·Cell Metabolism·Gregory D CarteeJuleen R Zierath
Oct 25, 2016·The Journal of Physiology·Sally SpendiffRussell T Hepple
Nov 4, 2016·Frontiers in Physiology·Rafael BarretoAndrea Bonetto
Jun 1, 2015·ESC Heart Failure·Nicole EbnerAnja Sandek
Aug 29, 2017·Journal of Cachexia, Sarcopenia and Muscle·Jacob L BrownNicholas P Greene
Nov 22, 2017·The Journal of Cell Biology·Wei-Ke JiHenry N Higgs
Jul 22, 2018·Essays in Biochemistry·Lisa TilokaniJulien Prudent
Jan 8, 2019·The Journal of Physiology·Jean-Philippe Leduc-GaudetGilles Gouspillou
Jun 14, 2019·Nature Communications·Giulia FavaroMarco Sandri
Aug 2, 2019·The Journal of Physiology·Vita SonjakRussell T Hepple

❮ Previous
Next ❯

Citations

Jul 16, 2020·The Journal of Physiology·Shivam Gandhi, Christopher G R Perry
Nov 11, 2020·International Journal of Molecular Sciences·Manuela Bozzi, Francesca Sciandra
Nov 25, 2020·The Journal of Physiology·Valentin Dablainville, Anthony M J Sanchez
Feb 8, 2021·Cell Calcium·Agnese De MarioCristina Mammucari
Dec 31, 2020·International Journal of Molecular Sciences·Vanina Romanello
Jul 15, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Julia María Torres-VelardeJosé Pablo Vázquez-Medina
Aug 8, 2021·International Journal of Molecular Sciences·Jean-Philippe Leduc-GaudetGilles Gouspillou
Jul 17, 2021·The Journal of Physiology·Maude DulacGilles Gouspillou
Dec 21, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chennan WuXia Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms