DrRRA: a novel response regulator essential for the extreme radioresistance of Deinococcus radiodurans

Molecular Microbiology
Liangyan WangYuejin Hua

Abstract

Two-component systems are predominant signal transduction pathways in prokaryotes, and also exist in many archaea as well as some eukaryotes. A typical TCS consists of a histidine kinase and a cognate response regulator. In this study, a novel gene encoding a response regulator (we designate it drRRA) is identified to be essential for the extreme radioresistance of Deinococcus radiodurans. DrRRA null mutant (we designate it MR) is sensitive to gamma-radiation compared with the wild-type strain. Transcriptional assays show that numerous genes are changed in their transcriptional levels in MR at exponential growth phase under normal or gamma-radiation condition. Most of them are related to stress response and DNA repair. Antioxidant activity assays exhibit that both superoxide dismutases and catalases are decreased in the mutant, whereas Western blotting assays show that RecA and PprA are also reduced in MR, verifying the microarray and quantitative real-time PCR data. Furthermore, pulsed-field gel electrophoresis assay demonstrates that deletion of drRRA results in the delay of genome restitution. These data support the hypothesis that DrRRA contributes to the extreme radioresistance of D. radiodurans through its regulatory role...Continue Reading

References

Oct 1, 1976·Archives of Biochemistry and Biophysics·H Misra, I Fridovich
Dec 6, 1996·The Journal of Biological Chemistry·E A Arn, J N Abelson
Jan 1, 1997·Progress in Nucleic Acid Research and Molecular Biology·A I Roca, M M Cox
Jun 19, 1998·Frontiers in Bioscience : a Journal and Virtual Library·P R BiancoS C Kowalczykowski
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jong-Il Kim, Michael M Cox
Jun 18, 2002·Plant Physiology·Ildoo HwangJen Sheen
Oct 26, 2002·Journal of Bacteriology·Ashlee M EarlJohn R Battista
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yongqing LiuMichael J Daly
Jun 14, 2003·Biochemical and Biophysical Research Communications·Yuejin HuaBinghui Shen
Apr 21, 2004·Luminescence : the Journal of Biological and Chemical Luminescence·Bing TianYuejin Hua
Sep 14, 2004·PLoS Biology·Dennis R HarrisJohn R Battista
Oct 2, 2004·Molecular Microbiology·Issay NarumiHiroshi Watanabe
Mar 7, 2007·Science Progress·Martijn BekkerKlaas J Hellingwerf
Mar 22, 2007·PLoS Biology·Michael J DalyJames K Fredrickson
Apr 17, 2007·Trends in Biochemical Sciences·Rong GaoAnn M Stock

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Citations

Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Dec 23, 2015·Biochemical and Biophysical Research Communications·Sun-Wook JeongSangyong Lim
Mar 8, 2016·International Journal of Radiation Biology·Liangyan WangYuejin Hua
Jun 15, 2014·Biochemical and Biophysical Research Communications·Chengzhi LiuYuejin Hua
Jun 10, 2015·Current Opinion in Microbiology·Yoshizumi Ishino, Issay Narumi
Jan 13, 2015·Current Microbiology·Liangyan WangYuejin Hua
Sep 23, 2014·PloS One·Amir Miraj Ul Hussain ShahYuejin Hua
Dec 15, 2015·Applied and Environmental Microbiology·Deepti AppukuttanSangyong Lim
Nov 17, 2015·Biochemistry. Biokhimii︠a︡·A A Agapov, A V Kulbachinskiy
Sep 30, 2020·Journal of Biomolecular Structure & Dynamics·Dhirendra K SharmaYogendra S Rajpurohit
Jan 31, 2017·Molecular Biotechnology·Liangyan WangYuejin Hua
Jun 4, 2016·The Journal of Biological Chemistry·Yogendra S RajpurohitHari S Misra

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