Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function

Proceedings of the National Academy of Sciences of the United States of America
Paramasivam NatarajanTodd R Graham

Abstract

Aminophospholipid translocases (APLTs) are defined primarily by their ability to flip fluorescent or spin-labeled derivatives of phosphatidylserine (PS) and phosphatidylethanolamine (PE) from the external leaflet of a membrane bilayer to the cytosolic leaflet and are thought to establish phospholipid asymmetry in biological membranes. The identities of APLTs remain unknown, although candidate proteins include the Drs2p/ATPase II subfamily of P-type ATPases. Drs2p from budding yeast localizes to the trans-Golgi network (TGN), and here we show that this membrane contains an ATP-dependent APLT that flips 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD) PS and PE derivatives from the luminal to the cytosolic leaflet. To assess the contribution of Drs2p to this activity, TGN membranes were prepared from strains harboring WT or temperature-sensitive alleles of DRS2 and null alleles of three other potential APLT genes (DNF1, DNF2, and DNF3). Assay of these membranes indicated that Drs2p was required for the ATP-dependent translocation of NBD-PS, whereas no active translocation of NBD-PE or NBD-phosphatidylcholine was detected. The specificity of Drs2p for NBD-PS suggested that translocation of PS would be required for the function of Drs2p in p...Continue Reading

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Citations

May 20, 2009·The Journal of Membrane Biology·Nathan C RockwellJeremy Thorner
Nov 10, 2009·Nature Cell Biology·Paramasivam NatarajanTodd R Graham
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Joost C M Holthuis, Tim P Levine
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Oct 13, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sara J C GoslineErnest Fraenkel
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiaoming Zhou, Todd R Graham
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryan D Baldridge, Todd R Graham
Jan 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ryan D Baldridge, Todd R Graham
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pei-Chin TsaiFang-Jen S Lee
May 5, 2009·The Journal of Biological Chemistry·Guillaume LenoirJoost C M Holthuis
Oct 22, 2010·The Journal of Biological Chemistry·Susanne BrydeJoost C M Holthuis
Oct 28, 2005·Molecular Biology of the Cell·Sonja ReinerRoger Schneiter
Nov 24, 2006·Molecular Biology of the Cell·Ke LiuTodd R Graham
Jan 19, 2007·Molecular Biology of the Cell·Edina Harsay, Randy Schekman
Jan 18, 2008·Molecular Biology of the Cell·Kenzi NakanoKazuma Tanaka
Feb 22, 2008·Molecular Biology of the Cell·Mika IkedaYasuyuki Igarashi
Dec 8, 2010·Journal of Biochemistry·Kazuma TanakaTakaharu Yamamoto
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Mar 23, 2011·PloS One·Christopher L BrettRajini Rao
Jan 5, 2011·Biological Chemistry·Rosa L López-MarquésThomas G Pomorski
Oct 1, 2013·Pflügers Archiv : European journal of physiology·Rosa L Lopez-MarquesThomas Günther Pomorski
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tal Soo HaDean P Smith
Jan 4, 2013·Cellular and Molecular Life Sciences : CMLS·Bruno MesminGuillaume Drin
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Jan 10, 2016·Biochimica Et Biophysica Acta·Cédric MontignyGuillaume Lenoir
Jan 12, 2012·Biochimica Et Biophysica Acta·Tessy T SebastianTodd R Graham

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