PMID: 9430764Feb 7, 1998Paper

Drug binding sites on chicken albumin: a comparison to human albumin

Journal of Veterinary Pharmacology and Therapeutics
H RajaianC J Bowmer

Abstract

Mammalian albumins have two main structurally selective ligand binding sites. Site I binds drugs such as azapropazone, phenylbutazone and warfarin; whereas benzodiazepines, some dansyl amino acids, such as dansylsarcosine, and short chain fatty acids like octanoic acid interact with site II. However, it is not known if non-mammalian albumins have similar binding loci. In this study, drug binding sites on chicken albumin were investigated using site selective fluorescent probes (warfarin and dansylsarcosine) and p-nitrophenyl acetate (NPA); the hydrolysis of which is selectively inhibited by site II ligands. Azapropazone and phenylbutazone decreased the binding of warfarin and dansylsarcosine to a similar extent. Diazepam and octanoic acid also inhibited binding of the two fluorescent probes in a non-selective manner. However, the fluorescence intensity of the warfarin-chicken albumin complex decreased when the pH was increased from 6.0-9.0; but by contrast, the fluorescence of bound dansylsarcosine remained unchanged. Furthermore, the hydrolysis of NPA was selectively inhibited by dansylsarcosine, diazepam and octanoic acid (ligands selective for site II on mammalian albumins), but not by site I selective ligands such as azapro...Continue Reading

Citations

Oct 16, 2008·Archives of Toxicology·Yan-Min Chen, Liang-Hong Guo
Oct 9, 2008·Analytical and Bioanalytical Chemistry·Paul HannewaldJean-François Muller
May 1, 2017·Journal of Pharmaceutical Sciences·Keishi YamasakiMasaki Otagiri
Aug 6, 2019·Journal of Biomolecular Structure & Dynamics·Mohd Aamir Qureshi, Saleem Javed
Oct 23, 2019·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Shouta M M NakayamaMayumi Ishizuka
Jul 1, 2006·Analytical Chemistry·Paul HannewaldJean-François Muller

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