Drug-delivering nerve conduit improves regeneration in a critical-sized gap

Biotechnology and Bioengineering
Pratima LabrooJayant P Agarwal

Abstract

Autologous nerve grafts are the current "gold standard" for repairing large nerve gaps. However, they cause morbidity at the donor nerve site and only a limited amount of nerve can be harvested. Nerve conduits are a promising alternative to autografts and can act as guidance cues for the regenerating axons, without the need to harvest donor nerve. Separately, it has been shown that localized delivery of GDNF can enhance axon growth and motor recovery. FK506, an FDA approved small molecule, has also been shown to enhance peripheral nerve regeneration. This paper describes the design of a novel hole-based drug delivery apparatus integrated with a polytetrafluoroethylene (PTFE) nerve conduit for controlled local delivery of a protein such as GDNF or a small molecule such as FK506. The PTFE devices were tested in a diffusion chamber, and the bioactivity of the released media was evaluated by measuring neurite growth of dorsal root ganglions (DRGs) exposed to the released drugs. The drug delivering nerve guide was able to release bioactive concentrations of FK506 or GDNF. Following these tests, optimized drug releasing nerve conduits were implanted across 10 mm sciatic nerve gaps in a BL6 yellow fluorescent protein (YFP) mouse model...Continue Reading

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Citations

Mar 21, 2020·Journal of Biomedical Materials Research. Part a·Qing LiDi Lu
Oct 3, 2019·Expert Review of Clinical Pharmacology·T M SaffariC A Hundepool
Mar 12, 2020·International Journal of Molecular Sciences·Damien P Kuffler, Christian Foy
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Apr 6, 2021·Neural Regeneration Research·Rui-Cheng ZhangDe-Qin Geng
Aug 22, 2021·Stem Cell Reviews and Reports·Liangfu JiangXiaofeng Jia

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