PMID: 6107337Oct 1, 1980Paper

Drug distribution as a function of binding competition. Experiments with the distribution dialysis technique

The Journal of Pharmacy and Pharmacology
M H Bickel, R Gerny

Abstract

In the distribution dialysis technique each of the two dialysis chambers contains a binding system, and a drug is allowed to distribute between them. This technique was tested by using various intracellular and extracellular binder preparations over wide concentration ranges, and model drugs selected for their known binding properties. The drugs were then tested at therapeutic concentrations in standardized systems of liver homogenate (0.5 g ml-1) and whole blood (0.02 ml ml-1). The resulting intracellular/extracellular concentrations ratios were characteristic for the binding properties of the various drugs. Thus, for imipramine, a drug with strong tissue and weaker plasma binding properties, the concentration ratios were 25 for the system homogenate/buffer, 0.8 for buffer/blood, and 15 for the competitive system homogenate/blood. In experiments with homogenates from various tissues (liver, lung, kidney, intestine, brain) and blood in the standard system, the following approximate ratios were obtained: 1 for antipyrine, 2 for phenylbutazone, 14 for imipramine (but only 8 with muscle, skin and adipose tissue). These results reflect both the individual binding to intracellular and extracellular components and the tissue/blood co...Continue Reading

References

Dec 1, 1977·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R Stegmann, M H Bickel
Jul 1, 1978·Clinical Pharmacology and Therapeutics·M GibaldiP J McNamara
Dec 1, 1978·Journal of Pharmacokinetics and Biopharmaceutics·L Z Benet
Jan 1, 1976·Drug Metabolism Reviews·W J Jusko, M Gretch
Mar 1, 1977·Chemico-biological Interactions·C D Francesco, M H Bickel
Jan 1, 1975·Annual Review of Pharmacology·G R Wilkinson
Mar 1, 1974·Chemico-biological Interactions·M H Bickel, J W Steele
Oct 1, 1970·Journal of Theoretical Biology·K B Bischoff, R L Dedrick
Aug 1, 1968·Biochemical Pharmacology·H YoshimuraH Tsukamoto
Aug 1, 1968·Journal of Pharmaceutical Sciences·K B Bischoff, R L Dedrick
Nov 1, 1957·The American Journal of Physiology·F BISCHOFF, R D STAUFFER

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Citations

Mar 20, 2008·European Journal of Drug Metabolism and Pharmacokinetics·C TesseromatisG Kostopanagiotou
Dec 7, 2005·Journal of Computer-aided Molecular Design·S R Mente, F Lombardo
Dec 17, 2011·European Journal of Drug Metabolism and Pharmacokinetics·Kotsiou AntoniaChristine Tesseromatis
Dec 17, 2004·Journal of Veterinary Pharmacology and Therapeutics·P L Toutain, A Bousquet-Mélou
Jan 1, 1989·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·W C Eckelman
Apr 1, 1981·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S Mühlebach, M H Bickel
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Michael J AvramThomas K Henthorn
Jul 1, 1983·The Journal of Pharmacy and Pharmacology·J R Castillo-FerrandoF De La Torre Brasas
Oct 27, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tristan S MaurerDennis O Scott
Oct 15, 1985·Biochemical Pharmacology·C Di Francesco, M H Bickel

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